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Health & Fitness

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST 2026

By fadilawati ali

ISCEP 2026 At Sunway Putra Hotel 17-18 August 2026

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Published
Aug 15, 2026
Pages
102
Language
English

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ABSTRACT

POSTER PRESENTATION ABSTRACT

P27

INTRINSIC TOXICITY OF DELTAMETHRIN AGAINST AN ADULT AEDES AEGYPTI LABORATORY STRAIN VIA TOPICAL BIOASSAY

Nur Amalina KAMARUDIN, Farah Diana ARIFFIN, Azahari ABDUL HADI, Shazreena ROSLI, Khairul Asuad MUHAMED, Beckham Tan Chia HUNG, Nurul Fatihah MOHAMAD JAAFAR, Lukman Hakim HAIRUDDIN, Nur Hazwani BAKARUDDIN, Nur Farihah AB HAMID, Rosilawati RASLI

Medical Entology Unfectious Disease Research Centre (DRC) shute for Medical Research (WR) National ones of Health pH Ministry of Health Malaysia (MOH) Setia Alas Selangor Malaysia

ABSTRACT

Determining the intrinsic response of an Aedes aegypti laboratory strain to deltamethrin via topical bioassays isolates insecticide toxicity from confounding behavioural factors. This approach quantifies toxicity by ensuring direct exposure and full absorption of insecticides. This study evaluated the intrinsic response of adult female Ae. aegypt to a range of deltamethrin concentrations. In addition, the fold concentration of deltamethrin was determined by comparing the diagnostic dosage with the World Health Organization (WHO) discriminating concentration of deltamethrin. Baseline concentrations were prepared from technical-grade deltamethrin (90% purity), ranging from 0.0005 mg/L to 2 mg/L across ten doses. Tested doses were directly applied to the thorax of anaesthetised adult female Ae. aegypt (laboratory strain, aged 3 to 5 days) using a calibrated micropipette. Mortality was recorded after 24 hours. and probit analysis was performed to determine the LD, and LD, values. A dose-dependent mortality increase was observed, with concentrations ranging from 0.0006 mg/L to 0.0008 mg/L produced low mortality 0.005 mg/L to 0.008 mg/L produced moderate mortality, and 0.05 mg/L to 0.08 mg/L produced high mortality indicating a suitable range for probit analysis (chi-square, X 16.04, p < 0.005). The analysis yielded an LD value of 0.08 mg/L (95% CI: 0.027 mg/L -0.508 mg/L) and an LD, value of 4.97 mg/L (95% CI:0.67 mg/L - 103374.46 mg/L). This study provides a baseline reference for establishing concentration series in topical bioassays assessing the intrinsic toxicity of deltamethrin against Ae. aegypti. The resulting LD, serves as a comparative benchmark for other insecticides, whilst the LD,, can inform discriminating dosage determinations.

Keywords: intrinsic topical bioassay probit analysis

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005