ISCEP 2026 At Sunway Putra Hotel 17-18 August 2026
ABSTRACT
ORAL PRESENTATION ABSTRACT
T2_06
RAPID DENGUE DETECTION OF TRANSOVARIAL TRANSMISSION IN AEDES VECTORS FROM CENTRAL JAVA PROVINCE, INDONESIA
Shamshul Anuar INTAN-UMAIRATH, Isa Mohamad RODI Sayono SAYONO, Mohd Shah NURUL-AZIRA", Didik SUMANTO', Othman WAN-NORAFIKAH**
Faculty of Medicine, Universi Teknologi MARATM Selangor Branch, Sungai Buon Campus Jalan H Sungai Buloh, Selangor Malaysia
HW ReNe Universis Teknologi MARA ATM Shah Alam Selang Masa Faculty of Public Healersitas Muradiyah Semarang Jalan Kadanganda Raye No Semarang Cental Java Province does
ABSTRACT
Dengue virus (DENV] can be transmitted through both horizontally and vertically within Aedes mosquito populations, potentially resulting in a dengue outbreak Vertical transmission specifically transovarial transmission, occurs when infected female mosquitoes transmit the virus to their offspring. This study aimed to determine the occurence of transovarial transmission of DENV among Aedes vectors collected from tourist attractions in Central Java Province, Indonesia Mosquito larvae were collected using ovitraps from seven tourist attractions, consisting of beaches, gardens, a heritage site and community parks. The collected larvae were reared under laboratory conditions and morphologically identified at the adult stage. First generation (F1) female adult mosquitoes of eight Aedes aegypti and eight Aedes albopictus populations, including laboratory strains as a reference, were grouped into pools of five individuals, yielding six pool per population. In total, 240 As. aegypti and 240 Ae. albopictus mosquitoes from 48 pools per species were subjected to rapid DENV detection using the Bioline Dengue NS1 Ag rapid test kit. All teated mosquito pools were negative for the dengue NS1 antigen (pool positivity rate=0%), indicating no detectable transovarial transmission among the tested populations. These negative findings could be attributed to low DENV transmission during the sampling period, low natural infection rates within the mosquito populations, or viral loads falling below the detection Emit of the NS1 rapid test kit. Although no positive samples were detected, the NS1 rapid test remains a beneficial tool for the field screening of dengue virus.
Keywords: Dengue NS1 antigen nopid detection, Andes angypt, Aedes albopictus Indonesia
INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005