INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST 2026

• ENTOMOLOGY
• PEST myOPO
• 15 POINTS
• INTERNATIONAL

SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST (ISCEP) 2026

INNOVATIVE ENTOMOLOGY FOR PUBLIC HEALTH, FOOD SAFETY AND URBAN SUSTAINABILITY

• SUNWAY PUTRA HOTEL KUALA LUMPUR, MALAYSIA
• AUGUST 2026
• Organized by:
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• MESSAGE
• Dr. Noraryana binti Hassan.
• Director Over
• Control Divisie istry of West Malaya
• Mrs. Khadijah Khairuddin.

Chaimue of the Organizing Camesittee of international Soiesse Conference co Entomology and Peat 2525

Mr. Mohd Khalil Junch...

President of PEKA

ABOUT MALAYSIAN ASSOCIATION OF PUBLIC HEALTH ENTOMOLOGY (PEKA)

ORGANISATION CHART OF PEKA 2025-2027

ORGANISING COMMITTEE OF THE INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST (ISCEP) 2026.

• PROFILES
• Keynote Speaker
• Plenary Speaker
• Invited Speaker
• PROGRAMME AT GLANCE
• 17 August 2006
• 18 August 2026
• LIST OF POSTER PRESENTATION.
• KEYNOTES ADDRESS.
• ABSTRACTS
• Plenary Speaker Sessions
• Invited Speaker Sessions

Oral Presentation - Innovations in Vector Management

Oral Presentation - Vector Surveillance Strategies..

Oral Presentation - Vector Susceptibility and Control Approaches...

• Poster Presentation.
• FLOOR PLAN/TRADE EXHIBITION.
• ACKNOWLEDGEMENTS.
• LIST OF DELEGATES..

FROM THE DIRECTOR DISEASE CONTROL DIVISION MINISTRY OF HEALTH MALAYSIA

DP Pay Yo

Assalamualaikum Warahmatullahi Wabarakatuh and Greetings...

It gives me great pleasure to extend my warmest congratulations to the Organising Committee for successfully comeaning the International Scientific Conference an Entomology & Peat 2026 (ISCEP 2026). I am honoured to welcome all delegates. distinguished speakers. researchers academicians, public health professionals, industry representatives, and students from Malaysia and around the world.

The theme of this year's conference, "Innovative Entomology for Public Health, Urban Sustainability, and Food Security aptly reflects the expanding role of entomology in addressing some of today's most pressing global challenges. Vector-borne diseases continue to place a significant burden an public health, while rapid urbanisation, environmental changes, and the growing demand for sustainable food production call for innovative, evidence-based approaches to pest and vector management.

Entomology remains an integral component of public health practice. Advances in surveillance systems, vector biology Insecticide resistance monitoring molecular diagnostics, digital technologies, artificial intelligence, and integrated vector management continue to strengthen our ability to prevent and control vector-bome diseases. At the same time, multidisciplinary collaboration is becoming increasingly important in developing practical and sustainable solutions

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006 that protect human health while preserving environmental integrity

ISCEP 2025 provides an excellent platform for scientific exchange, professional networking, and international collaboration. By bringing together experts from diverse disciplines and countries, this conference promotes the sharing of research findings, operational experiences, and innovative technologies that can be translated into effective public health policies and practices.

I would like to express my sincere appreciation to the Organising Committee, scientific committee, speakers, sponsors, exhibitors, and all supporting partners whose dedication and hard work have made this conference possible. Your collective efforts reflect a shared commitment to advancing scientific knowledge and strengthening regional and intemational cooperation

I wish every delegate a rewarding and productive conference, meaningful discussions, and continued success in advancing innovative entomological research for the benefit of public health, urban sustainability and food security. Thank you

Dr Noraryana binti Hassan Directer Disease Control Division Ministry of Health Malaysia

FROM THE CHAIRMAN OF THE ORGANIZING COMMITTEE OF INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST 2026

WELCOME ADDRESS BY ORGANISING CHAIRPERSON

On behalf of the Organizing Committee, it is my distinct honour to welcome all delegates, speakers, industry partners, and distinguished guests to the Intemational Scientific Conference an Entomology and Pest 2026 1SCEP 2026) at the Sunway Putra Hotel Kuala Lumpur. Reflecting on our joumey since our 2024 gathering at Bangi Resort Hotel, I express my sincere gratitude to the Malaysian Association of Public Health Entomology (PEKA) and our dedicated working committees for their vision and hard work in bringing this landmark symposium to life.

Hosted in the vibrant heart of Kuala Lumpur, our theme, "Innovative Entomology for Public Health, Food Safety, and Urban Sustainability underscores our shared imperative to embrace forward- thinking, sustainable strategies. Over the next two days, distinguished experts and participants from across the globe will share cutting-edge insights across our key pilars, spanning vector-bome disease surveilance, integrated vector control, sustainable urban peat management, and food safety with HACCP standards compliance Bringing together researchers, academicians, industry leaders, and students, ISCEP 2026 serves as a powerful beacon of collaboration and excellence in public health entomology I invite you to engage fully in our scientific program, faster anduring professional partnerships, and experience the warm hospitality and cultural diversity of Kuala Lumpur. May your time at ISCEP 2026 be both impactful and inspiring Thank you

Khadijah Khairuddin

Chaun of the Organising Canestee of international Science Conference on Enamelogy and Pest 2026

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

FROM THE PRESIDENT OF PEKA

My H

On behalf of the Malaysian Association of Public Heath Entomologist (PEKA), it is my great pleasure to welcome all of you to the International Scientific Conference on Entomology & Pest 2026 0SCEP 2026)

First and foremost, I would like to extend my heartfelt appreciation and sincere gratitude to each and every one of you for joining us at the International Scientific Conference on Entomology & Past (SCEP) 2026. Your presence reflects your commitment to advancing the field of entomology and pest management, and it is your support that makes this conference both meaningful and It is my great honour, as President of the Malaysian Association of Public Health Entomology (PEKA), to warmly welcome you all

Established in 2011, PEKA has grown into a professional platform that brings together experts from diverse disciplines within public health entomology in Malaysia Our membership comprises Science Officers (Entomology researchers academicians, lecturers, and professionals from the private sector, all united by a common vision to advance scientific knowledge and strengthen professional collaboration

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Since its inception, PEKA has remained committed to promoting the exchange of knowledge through conferences, seminars, workshops, and scientific meetings. These initiatives serve to strengthen collaboration, encourage innovation, and foster greater understanding among researchers, practioners, consultants, academics, government agencies, and industry partners. Guided by these objectives, we are proud to organize the International Scientific Conference on Entomology & Pest (SCEP) 2024, a two-day conference held here at the Sunway Putra Hotel, Kuala Lumpur, Malaysia. On behalf of PEKA, I would like to express my sincare appreciation to our organising committee, scientific committee, sponsors, partners, speakers, and volunteers for their dedication and hard work in making this conference possible

Finally, I extend my heartfelt thanks to all participants for your support and commitment to advancing the field of entomology. I wish you a productive, engaging, and successful conference. Thank you.

Mohd Khai Ausoh President of PEKA

ABOUT PEKA

MALAYSIAN ASSOCIATION OF PUBLIC HEALTH ENTOMOLOGY (PEKA)

The Malaysian Association of Public Health Entomology (PEKA) is a professional non-governmental organisation established in 2011 to advance the field of public health entomology in Malaysia. Initially founded through the collective efforts of Science Officers (Entomology) from the Ministry of Health Malaysia, PEKA has since evolved into a multidisciplinary professional association with members from government agencies, universities, research institutions statutory bodies, the private sectors, and other organisations involved in entomology and public health. Registered under the Registrar of Societies (ROS), Malaysia, PEKA serves as a national platform for professionals dedicated to the prevention and control of vector-borne diseases, urban pests, and other public health challenges associated with insects and pests.

⚫ PEKA is committed to strengthening the public health entomology profession by:

⚫ Promoting excellence in public health entomology and pest management.

Advancing research, innovation, education, and professional development

Facilitating scientific communication and knowledge exchange among researchers, practitioners, policymakers, and industry partners.

Organising scientific conferences, seminars, workshops, training programmes, and continuing professional development (CPD) activities.

Encouraging interdisciplinary collaboration between government agencies, academia research institutions, healthcare organisations, and the pest management industry

Supporting evidence-based approaches to vector surveillance, integrated pest management, and public health protection.

Enhancing public awareness on the importance of entomology in safeguarding human health and environmental sustainability

OUR ROLES

Over the years, PEKA has become an active professional body that promotes scientific excellence through educational programmes, technical workshops, scientific publications, networking opportunities, and collaborative inves

The association continues to support capacity building among entomologists and allied professionals while contributing to national efforts in disease prevention, vector control, urban pest management, food safety, and environmental health

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCP) 200

• President
• Tn Hj Mohd Khalil Jusoh
• Deputy President
• Mrs Noor Aslinda Ummi Awang Besar
• Secretary
• Mra Khadijah Khairuddin
• Asst Secretary
• Mc Mohd Amierul Fikri Mahmud
• Treasurer
• Mrs Norzihan Mohamed Hassan
• Asst Treasurer
• Mc Lim Cheng Cheong

Committee Member Mrs. Fadilawati Al Mrs Suraya Sulaiman

• Mc Shamsudin Abd. Rahman
• Mc Mohd Ridzuan Othman
• Mc Mohamad Zamri Mohamad Yusof

Mr. Mohd Faiz Mohd Shukry Mrs. Intan Nooraniza Anuar Assoc. Prof. Dr. Haster Salm

Assoc. Prof. Dr. Aishah Hani Azi

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

• President
• Tn Hj Mohd Khalil Jusoh
• Deputy President
• Mrs Noor Aslinda Ummi Awang Besar
• Secretary
• Mra Khadijah Khairuddin
• Asst Secretary
• Mc Mohd Amierul Fikri Mahmud
• Treasurer
• Mrs Norzihan Mohamed Hassan
• Asst Treasurer
• Mc Lim Cheng Cheong

Committee Member Mrs. Fadilawati Al Mrs Suraya Sulaiman

• Mc Shamsudin Abd. Rahman
• Mc Mohd Ridzuan Othman
• Mc Mohamad Zamri Mohamad Yusof

Mr. Mohd Faiz Mohd Shukry Mrs. Intan Nooraniza Anuar Assoc. Prof. Dr. Haster Salm

Assoc. Prof. Dr. Aishah Hani Azi

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

KEYNOTE SPEAKER

Professor Dr. Intan Ahmad

Professor Dr. Intan Ahmed is a Professor of Entomology at the School of Life Sciences and Technology Iratitut Teknologi Bandung (TB) Indonesia. He received his Bachelor's degree in Biology from ITB and a Ph.D. in Entomology from the University of Illinois at Urbana-Champaign, USA With a strong foundation in general biology, his expertise spans urban and structural entomology, public health entomology and industrial applications, with a particular focus on insecticide resistance and integrated pest management. Prior to joining ITB, he worked as a Senior Marketing Executive pesticides) at The Wellcome Foundation Ltd and as a Research Assistant in mosquito surveys at the incis Natural History Survey, USA At ITB, he has held several senior academic leadership positions, including Chair of the Academic Senate Dean of the School of Life Sciences and Technology and Dean of the Faculty of Mathematics and Natural Sciences

PLENARY SPEAKER 1

Professor Dr. Hidayatulfathi Othman

Professor De Hidayatulathi Othman holds a PhD in Biomedical Science of the Universiti Kebangsaan Malaysia, specializing in Medical Entomology. Author of over 106 publications, she is one of the Executive Members of the Southeast Asia One Health University Network, an ecutive Board member of the Malaysia One Health University Network, also an Ad-hoc sub-committee member for the Akademi Sains Malaysia to support activities and issues of climate change with a special focus on vector-bame doesses Currently, she is lecturer and researcher at Universiti Kebangsaan Malaysia, working closely with several agencies such Ministry of Health and municipalities. She and also as independent consultant to few insecticide and pest control companies. Her latest engagement is with UNICEF to apply the One Health concept of inter-ministerial and agencies collaboration on mitigating climate change effect and environmental degradation on child

Ms. Jo-Lynn Teh, BCE

Jo Lynn Teh, BCE, is Director of HYSIA Singapore, an environmental health company specialising in pest management, food safety and digitalisation. She is a Board- Certified Entomologist with the Entomological Society of America and a licensed Vector Control Technician under Singapore's National Environment Agency Additionally, Jo Lynn lectures on the Integrated Pest Management module at Republic Polytechnic, Singapons, and currently serves as Honorary Secretary for the Singapore Pest Management Association. With over 15 years of technical and operational expertise, Jo Lynn specialises in investigation, consulting, and designing advanced solutions beyond conventional pest management

PLENARY SPEAKER 3

Mr. Amran Hasan

Che Hasan

Mt Aman Hasan currently serves as the Deputy Director of Pre-Market Approval Food Safety and Quality Division, Ministry of Health (MOH) Malaysia. He is respon sible for managing certification activities including MeSTL, GMP, and HACCP, as well as other certification programa under the Food Safety and Quality Programme. He is also a certified GMP and HACCP auditor and actively involved in the development and capacity building of MOH certification auditors. He holds a Degree in Food Sci- ence and Technology from Universis Putro Malaysia and a Master of Science from the International blamic University Malaysia. With extensive experience in the Ministry of Health since 2005, he has served in several divisions including JKN Negeri Sembilan JKN Kelantan, JKN Pahang, and the Food Safety and Quality Control Division (FQCD) Putrajaya. His wide-ranging experience reflects his strong commitment to food safety and quality assurance in Malaysia

PLENARY SPEAKER 4

Mr. Ts. Mohd Azrool Rizal Mohd Azahari

Mt Ta Mohd Azrool Rizal Mohd Azahari is a Senior Assistant Director of Entomology at the Sabah State Health Department and a registered Allied Health professional and Professional Technologist (MBOT). With over 20 years of experience in medical entomology he specializes in vector surveillance, dengue and malaria control and public health innovation. He is the inventor of award-winning technologies, including the Mobile Fly Trap (MoFT) and the internationally downloaded Mo Kay mosquito identification app. His work integrates research, innovation, and community engagement to strengthen evidence-based vector control programma in Malaysia

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCP) 2005

• INVITED SPEAKER 1
• Dr. Nazni Wasi Ahmad
• INVITED SPEAKER 2
• Mr. Khalid Muhamad Salleh

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

De Nazi Wasi Ahmad is a Senior Research Officer at the Institute for Medical Research, Ministry of Health Malaysia, with over three decades of expertise in medical entomology, vector biology, and vector-bome disease control. Holding a Master of Science and a PhD from the University of Malaya, her research spara fly control forensic entomology maggot debridement therapy and innovative mosquito prevention strategies such as Wolbachia-based population replacement, Sterile Insect Technique (SIT) and autodissemination technologies. A leading figure in dengue vector control, Dr. Naani has served as Principal Investigator for 49 research projects funded by national and institutional grants, and she managed the Good Laboratory Practice-compliant vector control testing facility at IMR from 2016 to 2021 with support from the World Health Organization Internationally recognized, she contributed to WHO's Operational Manual on the Use of Wolbachia for Dengue Control, consulted on insecticide resistance monitoring, and served as Technical Officer for the IAEA Technical Cooperation Programme on SIT for Aedes suppression. She has also built global research partnerships with premier institutions, including the Universides of Glasgow, Melbourne, Texas, and Strathclyde. Over her distinguished career, Dr. N has published more than 200 peer-reviewed papers, delivered over 312 scientific presentations, co-obtained nine patents, and earned 46 national and international awards-including the prestigious WHO Dr Lee Jong-Wook Memorial Prize for Public Health for her innovative research in maggot debridement therapy

Mc Khalid Muhamad Salleh is an experienced public health pest control specialist serving as the Techno Commercial Manager at Foggers Marketing Sdn Bhd, where he leverages 26 years of industry experience to lead the management, marketing and in- house research and development of pest control equipment and pesticides. He holds a Bachelor of Applied Science with Honours in Parasitology from Universiti Sains Malaysia and is a certified Malaysian Pesticide License Applicator. Throughout his career, Mr. Khalid has provided expert consultation, troubleshooting, and specialised training for Pest Control Operators, government bodies, and the hospitality sector Additionally, he actively contributes to industry education as an official trainer for The Pest Control Operator (PCO), Pesticide Applicator License (PALL and Assistant Pesticide Applicator License (APAL) competency examinations under the Ministry of Agriculture and Food Security

• DAY 1 (MONDAY, 17 AUGUST 2026)
• MORNING SESSION
• Opening Ceremony & Keynote Address
• Registration of Delegates
• (Grand Ballroom Lobby)
• Arrival of Guests
• 7:30am- 8:30am
• 8.30am- 8.45am

Tuan Hj Mohd Khalil bin Jusch, President of PEKA

Dr Aminuddin bin Ma'pol, MOH Kepong

8.45am- 9.00am

Dr. Racita binti Ab Rahman, Deputy State Health Director (Public Health, WPKL & Putrajaya

Mrs. Viola binti Michael, Senior Deputy Director of Allied Health Science Division

Arrival of YBrs Dc Noraryana bin Hassan

Director Disease Control Division, Ministry of Health Malaysia

Singing of the National Anthem (Negaraku)

Prayer Recitation by Mr. Muhammad Al Azim Baharudin

Cultural Dance Performance

9.00am- 9.20

9.20am- 9.25am

Welcome Address by the Chairman of the Organising Committee of International Scientific Conference on Entomology and Pest 2026 Mrs Khadijah Khairuddin

OPENING SPEECH AND OFFICIATION OF INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY & PEST 2026

9.25am-

9.400m

YBrs Dr Dc Noraryona bin Hassan, Director Disease Control Division, Ministry of Health Malaysia

ISCEP 2024 Launch Gimmick & Montage Presentation

Presentation of Appreciation to Sponsors

1. Mr. Ling Hwel, Marketing Manager of ISQ

2. Mr Lee Teck Peng, Commercial Director of ENVU

3. Mr Kevin Cheong, CEO of ACM

• KEYNOTE ADDRESS
• 9.40m- 9.50
• Chairperson
• Assoc. Prof. Dr. Hasber Salim
• Universiti Sains Malaysia (USM)
• Keynote Speaker

Entomology and Public Health in a Changing World-Challenges and Innovations Professor Dr. Intan Ahmad

10:45am- 100am

Institut Teknologi Bandung (ITB), Indonesia

Photo session, Coffee Break, Networking Session, Visit to Exhibition Hall and Poster Booths Presentation of Token of Appreciation

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

• 11:30am
• PLENARY SPEAKER SESSION 1
• Chairperson
• Dr. Nurulhusna Ab Hamid
• Institute for Medical Research (MR)
• Plenary Speaker 1
• Iratitute for Medical Research (MR)
• INVITED SPEAKER SESSION 2

Vector-Borne Diseases and the Role of Insect Surveillance and Control Professor Dr. Hidayatullahi Othman

Universi Kebangsaan Malaysia (UKM), Malaysia

INVITED SPEAKER SESSION 1

The Waibachia Journey: Translating Research into Public Health Impact Dr. Nazni Wasi Ahmad

Termite Management Standards and the Current Practices of Termite Treatment in Malaysia

• Mr. Khalid Muhamad Salleh
• Foggers Marketing Sdn Bhd, Malaysia
• PLATINUM SPONSOR SESSION
• Agricultural Chemicals (M) Sdn Bhd
• 12.30pm-
• 12.50pm
• 12.50pm- 2:00pm

Lunch, Networking Session, Visit to Exhibition Hall and Poster Bootha

• EVENING SESSION
• PLENARY SPEAKER SESSION 2
• 2:00pm-
• Chairperson
• 2:30pm
• Mr. Mohd Haziq Asyraf Mohd Hamdan
• Pestwest Electronics Lisited Australa
• Plenary Speaker 2

Reshaping Urban Pest Control for Sustainable Cities

Ms. Jo-Lynn Tah

HYSM, Singapore

Innovations in Vector Management: Oral 1 - Oral 9

2:30pm- 2:46pm

ORAL PRESENTATION 1

STV03: A Rapid 3-Hour Trap for Adult Aedes Surveillance Ms. Atiqah Nor Ain

(Batu Pahat District Health Office, Ministry of Health, Malaysia)

2:45pm- 3:00pm

ORAL PRESENTATION 2

Optimising The Rearing of Wolbachia-infected Aedes aegypti From Challenges to Successful Mass Production

Mrs. Rafidah Ali

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

(Kuala Lumpur & Putrajaya Federal Temitory State Health Office, Ministry of Heal Malaysia)

• 3:15pm- 3:30pm
• 3:30pm- 8:45pm 8:45pm-
• 4:00pm
• ORAL PRESENTATION 3

Zero-Fly Strategy in Entomological Risk Management for VVIP Official Event Operations

Mrs. Zuraidah Ahmmad

Abu

(Batang Padang District Health Office, Ministry of Health, Malaysia)

ORAL PRESENTATION 4

Integrating Molecular Ecology for Sustainable Insect Pest Management Prol. Ta. Dr. Abdul Hafiz Ab Majid

(Universi Sains Malaysia-USM)

Coffee Break, Networking Session, Visit to Exhibition Hall and Poster Booths

ORAL PRESENTATION 5

Evaluation of Two Probe-Based qPCR Master Mises Performance for Filarial DNA Detection in Mosquito Vectors

• 4:00pm- 4:15pm
• Mrs. Rezki Sabrina Masse
• (Institute for Medical Research-R)
• ORAL PRESENTATION 6

Efficacy of Methoprene and Pyriproxylen on Glyphosate-Exposed Aedes Larval Populations

• 4:15pm- 4:30pm
• Mrs. Lailatul Nadhirah Aari
• (Universiti Teknologi MARA - WTM)

ORAL PRESENTATION 7

Preliminary Evaluation of a Low-Cost Ceramic-Based Artificial Blood Feeding System for Aedes aegypti Colonisation in Insecticide Resistance Testing Ms. Manimegalai A/P Varatharaja

(Kuala Lumpur & Putrajaya Federal Territory State Health Office, Ministry of Health, Malaysia) ORAL PRESENTATION 8

Methodology in Developing a Novel Integrated Malariogenic Potential Index (IMP) for Measuring the Risk of Plasmodium knowlesi Malaria Transmission

• 4.30pm- 4.45pm
• 4:45pm- 5:00pm
• 7:00pm-
• 10:30pm
• Mr. Tarrang Yusin

(Sabah State Health Office, Ministry of Health, Malaysia)

ORAL PRESENTATION

IPPM-R: A Smart Rodent Monitoring and Management System Using NFC, QR Code, and lot Technologies

• Dr. Mohd Kamir Yusof
• (Universiti Sultan Zainal Abidin - UniSZA)
• ISCEP 2026 Gala Dinner
• Grand Ballroom Sunway Putra Hotel
• Invited Guests)
• END OF DAY 1

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

• MORNING SESSION
• Arrival and Registration of Participants
• 7:30am- 8:30am
• (Grand Ballroom Lobby)
• PLENARY SPEAKER SESSION 3
• 8:30am-
• 9:00am
• Chairperson
• Assoc. Prof. Dr. Aishah Hani Azil
• Universiti Kebangsaan Malaysia (UKM)
• Plenary Speaker 3

Pest Management in the Context of Food Regulations and HACCP Integration

Mr. Amran Hasan @ Che Hasan

Ministry of Health (MOH), Malaysia

Vector Surveillance Strategies: Oral 10 - Oral 16

ORAL PRESENTATION 10

9:00am- 9:15am

Strengthening Border Health Security Through Integrated Vector Surveillance: Evidence from Pengkalan Kuber International Point of Entry, Malaysia (2023-2025) Mrs. Syarifah Nadwah Md Yunus

(Tumpat District Health Office, Ministry of Health, Malaysia)

9:15am- 9:30am

ORAL PRESENTATION I

Molecular Characterisation of the Blood Feeding Source of Anopheles maculatus in a Plasmodium knowlesi Transmission Area

Mr. Abdul Jabir Jaafar (Institute for Medical Research (MR)

9:30am- 9:45am

ORAL PRESENTATION 12

Community Diversity and Predictive Distribution of Japanese Encephalitis Virus Vectors Across Sabah Land Types

Mr. Ag Shazmeer Ag Safreeb (Universi Malaysia Sabah - UMS)

9:45am- 10:00am

ORAL PRESENTATION 13

Molecular Evidence of Orientia tsutsugamushi Circulation in Chiggers and Small Mammals from Human Scrub Typhus Hotspots in Pahang, Malaysia

Dr. Erniener Faralian Che Lah (Institute for Medical Research - MMR) ORAL PRESENTATION 14

Winged Guests in the Ward: Recurring Dipteran Infestations in a Public Hospital Intensive Care Unit

• 10:00am- 10:15am
• 10:15am- 10:45am 10:45am- 11:00am
• 11:00am- 11:15am
• Ms. Kalaivaani Adakalam
• ORAL PRESENTATION 15
• ORAL PRESENTATION 16

(Selangor State Health Department Ministry of Health, Malaysia)

Coffee Break, Networking Session, Visit to Exhibition Hall and Poster Booths

Rapid Dengue Detection of Transovarial Transmission in Aedes Vectors from Central Java Province, Indonesia

Ms. Intan Umairath Shamshul Anuar (Universiti Teknologi MARA-LOTM)

Uncovering Hidden Chigger Fauna in Beaufort, Sabah, Malaysia: First Malaysian Record of Schoengastia pseudoschaffneri and Baseline Data for Scrub Typhus Surveillance

Mrs. Suhail Zainal Abidin (Institute for Medical Research -MAR)

Vector Susceptibility and Control Approaches: Oral 17-23

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

ORAL PRESENTATION 17

Evaluation of Vectobac" WG a Water-Dispersible Bacterial Larvicide Formulation, for the Control of Anopheline and Culicine (Order: Diptera) Mosquito Vectors in Sarawak, Malaysia

• 11:30am- 11:45am
• 11:45am- 12:00pm
• Mr. Real Bohari

(Sibu Divisional Health Office, Ministry of Health, Malaysia)

ORAL PRESENTATION 18

Effectiveness of a Commercial Organophosphate Temephos Against Aedes albopictus Larvae from Residential Sites in Peninsular Malaysia

Ms. Nureen Afrina Nooruddin

(Universiti Teknologi MARA-UNTM)

ORAL PRESENTATION 19

Resistance Profiling of Aedes aegypti Using Intensity Concentrations Enhances the Evaluation of Insecticide Effectiveness for Vector Control

Dr. Rosilawati Rasli (Institute for Medical Research -MR)

• 12.00pm- 12:16pm
• 1216pm- 12:30pm
• ORAL PRESENTATION 20

ORAL PRESENTATION 21

Involvement of Detoxification Enzymes in Insecticide Resistance Among Aedes albopictus from Agronomical Areas of West Malaysia

Dr. Wan Norafikah Othman (Liniversiti Teknologi MARA-LATM)

Assessment of Fogging Machine Spray Distance and Its Effects on Vehicle Surfaces Ma. Farah Diana Ariffin (institute for Medical Research- MMR)

12:30pm- 12:45pm

ORAL PRESENTATION 22

Evaluation of Insecticide Resistance in Aedes aegypti from Kuala Terengganu for Sustainable Vector Control

Ms. Nur Sofea Kamelia Djafar (Universiti Malaysia Terengganu-UMT)

• 12:45pm- 1:00pm
• ORAL PRESENTATION 23
• 1:00pm- 2:00pm
• PLENARY SPEAKER SESSION 4
• 2:00pm-
• 2:30pm
• Chairperson
• Plenary Speaker 4
• 2:30pm 3:30pm
• 3:30pm- 5:00pm
• CLOSING CEREMONY

Evaluating the Residual Efficacy of Different Insecticide Formulations against Mosquito Vectors

Dr. Nurulhusna Ab Hamid (institute for Medical Research - MMR)

Lunch, Networking Session, Visit to Exhibition Hall and Poster Booths

EVENING SESSION

Dr. Eminar Faraiana Che Lah Iratitude for Medical Research (MR)

Innovation in Insects' Hygiene & Food Safety in Storage & Supply Chains Mr. Ts. Mohd Azool Rizal Mohd Azahari Ministry of Health (MOH), Malaysia

GOLD SPONSOR SESSION - ENVU

GOLD SPONSOR SESSION-ISQ Holdings Sdn Bhd SPONSOR SESSION - Compesti Sdn Bhd

Coffee Break, Networking Session, Visit to Exhibition Hall and Poster Booths

END OF DAY 2

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCP) 2005

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10. Jalan Industri USJ 14, TPS Taman Perindustrian USJ1 47600 Subang Jaya. Selangor Darul Ehsen

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• FIELD IMPLEMENTATION
• OXO

Assessing the Relationship Between Aedes Indices and Breeding Containers in Dengue Hotspots: Evidence from Lembah Pantai, 2023-2025

P01

Mrs. Noorazin Mohd Ali

(Lebah Partai District Health Office, Ministry of Hou

Population Dynamics of Anopheles introlatus and Its Potential Role in Zoonotic Malaria Transmission in Hutan Simpan Sempam, Raub, Pahang, Malaysia Mrs. Nurhanani Ibrahim

(Bentong District Heath Office Ministry of Health Malays

• PO2
• PO3
• Assoc. Prof Dr Aishah Hani Azil

(Kebangan

Integrating Gemini Gems Al to Enhance Mosquito Identification in Higher Education: The HexaVector UKM Application

Distribution of Anopheles Vector in a Plasmodium knowlesi Risk Area in Hutan Simpan Kahang, Kluang, Johor, Malaysia

• PO4
• P05
• Mrs. Fadilawati Ali

(Kang District Huth Of My of Mrs. Khadijah Khairuddin

(Pling District Health Office Ministry of Health Maly

Empowering Communities to Break the Dengue Transmission Chain in Selangor, Malaysia (2018-2026)

Small Fies, Big Risks: Tackling Drosophila Infestation in a Healthcare Facility in Kota Setar, Kedah

P06

Mrs. Nurul Farhanah Mohd Nasir

(Kara Star Distict Health Office, Ministry of Health Malaysia)

Enhancing the Efficacy of Rodent Control in a Healthcare Facility in Southwest Penang Through Multi-Intervention Strategies: Findings from October 2024 to May 2026

PO7

Ms. Wong Oi Chen

(Southwest Distict Health Department Ministry of Hea

INTERNATIONAL SCIENTIFIC CONFERENCE ON

ENTOMOLOGY AND PEST SCEP) 2006

• PO8
• P09
• Mr. Mohd Amierul Fieri Mahmud

Mrs. Norzawati Youp

The Frontline Defence: Mapping the Adoption of Four Key Community-Led Mosquito Prevention Measures in Malaysia

Protecting the Milestone: Risk Mapping for Malaria Re-Introduction in Post- Elimination Malaysia

Assessment of Post-Outbreak Dengue Vector Density Using the Sticky Ovitrap Index (SOI) in Village Localities of Melaka Tengah District, Melaka

Ms. Sharifah Nur Shahirah Syed Abdul Hamid

P10

Disrupting the Cycle: Field Efficacy and Entomological Evaluation of the Aedes Tech Mosquito Home System (AMHS) in Johor Bahru, Malaysia

P11

Ms. Siti Saodah Ibrahim

Johor Bahru District Health Office, Ministry of Health

Lymphatic Filariasis Vectors in Southeast Asia: A Scoping Review of Research Gaps and Methodological Trends

• P12
• Mr. Mohd Amiru Hariz Aminuddin
• Bute for Public Health - PH
• Der of Poster Presen

Unveiling Perpetrator Behavior: Differential Fly Colonization on Dismembered Human Remains in Malaysia

P13

Dr. Syamsa Rizal Abdullah

The Power of Sticky Traps: Breaking the Dengue Transmission Chain via Adult Aedes Mortality

• P14
• P15
• Mrs. Khuzaimah Mohamad Sahib
• Mrs. Maziah Zakariye

Kota Tinggi District Health Office Ministry of Health

Unmasking Cryptic Breeding: Integrated Entomological Interventions in a Prolonged Dengue Hotspot at Perumahan PGA Kluang

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

P16

Mrs. Nur Kid Mohd Azmi

(Tanah Merah District Health Office, Ministry of H

Abundance, Diversity, and Distribution of Anopheles Mosquitoes Along the Malaysia-Thailand Border, Jeli District, Kelantan, Malaysia, 2018-2024

Deciphering Climate-Vector Interactions: Temperature and Rainfall Effects on Aedes Ovitrap Positivity and Oviposition Activity in Bandar Baru Pasir Puteh, Kelantan, Malaysia

• P17
• P18
• P19
• Poster Presenc
• P20
• Mrs. Suraya Sulaiman

(Pasi Pash Dinic Heats Office Ministry of Healt

• Ms. Intan Adibah Salim
• (Malay - LM
• Ms. Fatin Nur Adina Rozaidi

Buzzing Towards Danger Poultry Farms as Drivers of Public Health Risk Mrs. Noorazyan Haris

(Segamat District Health Office Ministry of Health Maly

Efficacy and Residual Activity of Pyripraxyfan Against Anopheles cracen Implications for Zoonatic Malaria Vector Control in Malaysia

Evaluating the Residual Efficacy and Strain-Dependent Persistence of Bifenthrin Surface Treatments Against Aedes aegypt

Evaluation of Different Rodenticide Baits for Rodent Management in CBU and PIDM Vehicle Storage Areas at Tanjung Malim, Perak

P21

Mrs. Nor Syazana Arif

(TP Conal Sin)

Genetic Diversity of Aedes albopictus in Kuala Terengganu Using COXI Gene Sequence for Sustainable Vector Control

• P22
• Ms. Intan Balqis Mohd Satar
• (Univers Malaysia Teng-UMT

Who's Buzzing in The Wetland? A Three-Year Longitudinal Analysis of Mosquito Fauna (2023-2025)

P23

Mrs. Hanipah Shahar

Kuala Lumpur & Punajaya Federal Tetory State Health Off

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Pollination-Based Flower Morphology Matching with Stingless Bee and Vanillin Content Analysis in Cultivated Vanilla Species

P24

Me.Qutine femaludin

(Universiti Malaysia Terengganu - UMT)

Comparative Developmental Stability of Chrysomya nigripes and Boettcherisca highlandica in Long-Term Laboratory Colonies: Implications for Forensic Chronology, Public Health, and Urban Sustainability

• P25
• Mr. Hilarian Mojiun
• As for Medical R-BR)

Entomological Risk Assessment (ERA) for Dengue Infection in Cheras District Public Places (2024-2025)

P26

Mrs. Nor Aszina lumal

Lagu & Putrajaya Federal Titary State Office Mistry of Intrinsic Toxicity of Deltamethrin Against an Adult Aedes aegypti Laboratory Strain via Topical Bioassay

P27

Mrs. Nur Amalina Kamarudin for Medical Reach-IMR)

Der of Poster Presen

Vector Competence of Aedes albopictus in Virus Transmission: A Systematic Literature Review

• P28
• P29
• Mrs. Raziah Ali
• And for Medical Reach-IMR)

Ma. Aimi Afiqah Mohammadin for Medical - BMR)

Comparison of the Residual Efficacy of Lambda Cyhalothrin Capsule Suspension against Aedes aegypti between Two Spray Cycles

Laboratory Observations on Oothecal Formation and Nymphal Emergence in German Cockroaches (Blattalla germanica)

• P30
• P31
• Ms. Ezita Qaisara Ridzuan

But for Medical Peach-IMP)

Comparative Residual Efficacy of Three Deltamethrin Formulations against Laboratory and Wild Strains of Aedes aegypti Under Semi-Field Conditions Ma. Norlaili Marawi

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

KEYNOTE SPEAKER

ENTOMOLOGY AND PUBLIC HEALTH IN A CHANGING WORLD-CHALLENGES AND INNOVATIONS

Apar

Intan AHMAD

School of Life Science and Technology Institut Teknologi Bandung (ITB) Fota Bandung Ja Bar Indonesia

ABSTRACT

Insects are among the most evolutionarily successful organisms on Earth, providing essential ecological services such as pollination, decomposition, and nutrient cycling that sustain biodiversity, agriculture, and human society. Although only a small proportion of insect species become pests or disease vectors, these highly adaptable species increasingly influence public health, food safety, and urban sustainability. Urbanisation, climate change, globalisation, and increased human mobility are reshaping insect-human interactions, creating new challenges for vector-bome diseases, food contamination, and structural pest management, particularly in Southeast Asia. The growing emergence of insecticide resistance demonstrates that technological interventions alone cannot overcome the evolutionary capacity of insects to adapt under continuous selection pressure. Drawing on research in insecticide resistance, urban pest management, vector control and digital surveillance, this keynote demonstrates how evolutionary thinking can guide practical innovation. Case studies include optimisation of the Sterile Insect Technique (SIT) for Aedes aegypti using biologically informed irradiation protocols and the development of IoT-enabled smart ovitraps with automated image analysis for real-time surveilance and evidence-based decision-making. These examples show that technological advances achieve their greatest impact when grounded in ecological and evolutionary principles. Building upon the concepts of Integrated Pest Management (PM) and Integrated Vector Management (IVM), this keynote advocates a systems-based approach integrating evolutionary biology ecology public health, food safety, digital technologies, governance, and evidence- based policy. Ultimately, successful innovation in public health entomology depends not only on new technologies but also on a deeper biological understanding of the insects being managed.

Keywords: public health entomology, insecticide resistance, IPM, M, Stare Insect Technique, digital surveilance

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

PLENARY SPEAKER 1

VECTOR-BORNE DISEASES AND THE ROLE OF INSECT SURVEILLANCE AND CONTROL

Hidayatulfathi OTHMAN

Centre for Toxicology and Health Rak Studies Faculty of Health Sciences Unkara Kahargaan Malaysia Kuala Lumpur, Malaysia

ABSTRACT say

Vector-borne diseases (VBDs), perscularly mosquito-bome viral and parasitic infections, remain among the world's most persistent public health challenges. Diseases such as dangus, chikungunya, Zika, and malaria continue to impose substantial health and economic burdens, particularly in tropical and subtropical regions. As environmental change, urbanisation, and global travel reshape disease transmission, effective entomological surveillance and adaptive vector control have become more important than ever Over the past decade, vector surveillance has evolved from conventional trapping and manual identification to intelligent, technology-driven systema. Artificial intelligence (AIL the Internet of Things (IoT) drones, robotics, genomic surveillance, and acoustic mosquito recognition now enable real-time monitoring of vector populations and environmental conditions supporting earlier detection of disease risks and more targeted interventions. Vector control strategies have also advanced considerably Biological approaches such as Wolbachia-based interventions. the Sterke and Incompatible Insect Techniques (SIT/IT), Al-assisted drone applications, attractive targeted sugar baits (ATSBS), and emerging gene-editing technologies are expanding the toolbox for Integrated Vector Management (MM) Southeast Asia, where dengue remains hyperendemic, has become a leader in implementing these innovations Singapore has pioneered IIT using Al-based male mosquito sorting, while Indonesia, Vietnam, and Malaysia have adopted Wolbachia-based population replacement strategin against Aedes aegypti. In Malaysia, nationwide Walbachis releases have shown encouraging reductions in dengue transmission, complemented by loT-enabled smart ovitraps for continuous surveillance and dengue hotspot mapping. For malaria, integrated vector management continues to evolve through targeted residual spraying, strengthened surveillance, and the introduction of RTS,S/AS01 and R21/Matrix-M vaccines in endemic settings. Together intelligent surveillance and innovative biological, environmental, and chemical interventions are reshaping vector control and strengthening our capacity to prevent vector-bome diseases, supporting sustainable public health and global health security.

Keywords: vector-borne diseases, insect surveillance, vector control, technology-driven systems, Integrated Vector Management (VML intelligent surveillance

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

PLENARY SPEAKER 2

RESHAPING URBAN PEST MANAGEMENT FOR SUSTAINABLE CITIES

Jo-Lynn TEH

ABSTRACT

Rapid urbanisation, climate change, and increasing human-pest interactions are intensifying pest risks in cities, affecting public health, food safety, and urban liveability. Conventional pest control has traditionally relied on reactive, treatment-focused approaches that target visible infestations rather than the underlying drivers of pest problems. Although effective in the short term, these practices often lead to recurring infestations, increased chemical use, pesticide resistance, and unintended environmental impacts. This presentation highlights the need to transform urban pest management from episodic treatment to risk-based, prevention-first systems founded on entomological science, ecological principles, and measurable performance outcomes. Integrated Pest Management (PM) and Pest Risk Management (PRM) provide the framework for this transition when consistently implemented in practice. A key example is Singapore Standard SS 721:2025 Code of Practice for the Performance of Pest Management Services. As Southeast Asia's first national performance-based pest management standard, SS 721 promotes structured pest risk assessment, proportionate interventions, continuous monitoring. and reduced dependence on routine chemical treatments. Developed through multi-stakeholder collaboration, it shits accountability from treatment frequency to measurable pest risk reduction while supporting broader public health and sustainability goals. The presentation will discuss how SS 721 operationalises IPM and PRM principles, how similar frameworks can be adapted to other urban settings, and how digital monitoring, data-driven decision-making, and cross-sector collaboration strengthen preventive pest governance. By positioning pest management as an integral component of urban sustainability, cities can better protect public health, improve food safety, and build resilience against growing ecological and climatic challenges.

Keywords: urban peat management, Integrated Pest Management, Peat Risk Management,bility55 721-2005, urban health

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

PLENARY SPEAKER 3

PEST MANAGEMENT IN THE CONTEXT OF FOOD REGULATIONS AND HACCP INTEGRATION

Amran HASAN @ CHE HASAN

Food Safety and Quality Program Ministry of Health Malay Malay @mohgowy

ABSTRACT

Pest infestation remains a significant challenge in food production environments because it can introduce biological, physical, and chemical hazards that compromise food safety. In Malaysia, pest management in food premises is regulated under the Food Act 1983 and the Food Hygiene Regulations 2009, requiring food businesses to implement preventive measures and maintain hygienic conditions. This paper examines the role of pest management within the Malaysian regulatory framework and its integration into Good Manufacturing Practice (GMP) and Hazard Analysis and Critical Control Paint (HACCP) systema, with reference to Malaysian Standard MS 1514 [GMP for Food) and Malaysian Standard MS 1480 (HACCP System) Audit findings from HACCP, GMP, and MeSTI certification programmes conducted by the Ministry of Health Malaysia identified recurring non-conformities affecting food safety management. Common findings included deficiencies in process flow diagrams and hazard identification during HACCP audits, as well as facility layout, infrastructure, cleaning and sanitation, and pest control programmes during GMP audits. MeSTI audits similarly highlighted weaknesses in premises hygiene, facility maintenance, pest control procedures, and food handler training. These findings demonstrate that effective pest management depends on strong prerequisite programmes rather than pesticide application alone.The paper highlights Integrated Pest Management (IPM) as a preventive approach based on facility design, sanitation, monitoring, corrective actions, verification, and continual improvement. Aligning regulatory requirements with GMP and HACCP enables food businesses to minimise contamination risks, strengthen food safety assurance, achieve regulatory compliance, and protect public health

Keywords: pest management, Food Act 1983, Food Hygiene Regulations 2009, GMP, HACCP. integrated pest management (PM)

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

PLENARY SPEAKER 4

INNOVATION IN INSECT HYGIENE AND FOOD SAFETY IN STORAGE & SUPPLY CHAINS

Mohd Azrool Rizal MOHD AZAHARI

Entomology & Past Sector Sabah State Health Departent Kota Kinabalu, Sabah, Malay marcelah gaviny

ABSTRACT

Global food supply chains are becoming increasingly complex due to rapid urbanisation, cimate change, expanding international trade, and growing consumer expectations for safe and sustainable food. These challenges have transformed insect hygiene from a conventional pest control activity into a strategic component of Food Safety Management Systema (FSMS). This plenary lecture reviews current and emerging innovations in insect hygiene across food production, storage, processing, and distribution, highlighting their role in preventing food contamination and strengthening supply chain resilience Major food-associated insects, including filth fies, cockroaches, and stored-product insects, are discussed together with their contamination pathways and impacts on food safety. public health, and regulatory compliance. Emerging technologies such as artificial intelligence (All computer vision, the Internet of Things (IoT), smart traps, Geographic Information Systems (GIS), predictive analytica, blockchain-enabled traceability, and digital compliance platforms are presented alongside sustainable innovations including biological control, RNA interference (RNAL semiochemicals, nanotechnology, and Integrated Pest Management (PM). The lecture concludes by introducing the Smart Integrated Insect Hygiene Intelligence Ecosystem (SIHIE), a framework integrating environmental intelligence, biological surveillance, digital technologies, predictive decision support, regulatory compliance, and sustainability to support proactive insect hygiene management and future-ready food supply chains

Keywords: artificial intelligence, food safety, insect hygiene, integrated pest management (PM), stored-product insects, supply chain

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

INVITED SPEAKER 1

WOLBACHIA MALAYSIA: TRANSLATING RESEARCH INTO PUBLIC HEALTH IMPACT

Nazni WA", Cheong YL, Farah DA', Kamarul MRG, Arif MAK, Zatil AMZ, Nizam MN, Irwan MS', Lee HL, Ami Fazlin SM, Wan MK, Perada WP, Khadijah K, Norazman MR, Hanipah S', Hoffmann A', Sinkins S*

Medical Entomology Unit Infectious Disease Research Cenee site for Medical Research Kala Lumpur Mys Breedical Mysere Unit Special Resource Cente, este for Medical Research Kuala Lumpur Malaysia se for Medical Research National sites of Health Ministry of Health Malesia Shab Alan Selangor Malaysia Vector Borse Disease Control Section Disease Contol Division Ministry of Health Malesia Pa Malesia Enology and Pest hit Vector Borne Disease Control Laboratory Selangor State Health Department King Selangor Malaysi Heath Department of Federal Tentory of Kle Lamp & Pak Jalan Cenderaan Kuala Lumpur Malaysia Pest and Ennstal Research Group School of BioSciences The University of Melbesme Parkle VIC 3310 Au MRC-University of Glasgow Centre for Virs Research Osgowe UK

ABSTRACT

Atstated Speaker

Malaysia has pioneered the implementation of the Wolbachis population replacement strategy as an innovative and sustainable approach for dengue contral in a tropical, dangue-andemic setting. The programme began in 2016 as a research initiative led by the Institute for Medical Research (MRL, Ministry of Health Malaysia, with community engagement conducted by the Institute for Health Behavioural Research (IHBR). Following successful stakeholder engagement, the first release of wbB-infected Aedes aegypti mosquitoes was undertaken in Kuala Lumpur in March 2017 The wAlbB strain was selected for its superior heat tolerance and strong dengue virus-blocking capacity under tropical conditions. Extensive Seld evaluations demonstrated stable Wolbachia establishment, persistence, and substantial reductions in dengue transmission providing the evidence for nationwide operationalisation by the Ministry of Health Malaysia in 2019. To date, the programme has expanded to 71 dengue hotspot localities across nine states. comprising 18 research and 53 operational sites, protecting approximately 313,000 residents. Long-term surveillance has confirmed sustained Wolbachia establishment, with intervention sites reporting 60-80% reductions in dengue incidence following implementation. As the programme expanded, IMR continued to provide scientific leadership through research, innovation, and technical oversight, while large-scale mosquito production was transitioned to a public-private partnership model to support sustainable implementation. Ongoing research includes the evaluation of Wolbachis-infected Aedes albopictus to broaden vector coverage and further optimise dengue control strategies. Despite these achievements, dengue remains a major public health challenge in Malaysia. As of Epidemiological Week 28, 2026, 42.848 dengue cases and 34 deaths were reported, representing increases of 348% and 78.9%, respectively. compared with the same period in 2025. With more than 60% of dengue cases occurring in the Kang Valley, expanding Wolbachia implementation across this highly endemic urban region is essential to maximise population-level impact. Malaysia's programme demonstrates how scientific innovation, strong community engagement, and multisectoral collaboration can successfully translate research into sustainable public health practice while providing a scalable model for other dengue-endemic countries.

Keywords: Wolbachia, Aedes aegypti dengue hotspots, population replacement, translational research, operationalisation

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

INVITED SPEAKER 2

TERMITE MANAGEMENT STANDARDS AND THE CURRENT PRACTICES OF TERMITE TREATMENT IN MALAYSIA

Khalid MUHAMAD SALLEH

Foggers Marketing Sah Bd18, Jalan Bukit Penal U2 Cheras ser Kale Lamp Selangor Malaysi [email protected]

ABSTRACT

Termites have always been an important economic pest in the world of structural pest control, contributing to a major share of profit, The PCOS have gone through a lot of changes both from pesticides and pesticide application techniques in termite management, MS 1848:2006 -Protection of buildings agairat subterranean termites Code of Practice for prevention, detection and treatment of infestation is a standard that have been initiated and formalised by Department of Standards Malaysia and align with the guidelines of the Malaysian Past Management Association (MPMA previously known an PCAM). It formalizes the guidelines on the various termites, the PCO's termite management ie. inspection, treatment regime using liquid and baiting and the termiticides at that point in time. This standard is intended for the Malaysian pest control industry and the methods for termite prevention and reinfestation. The industry has gone through rapid changes especially with the advance of latest technology. They have move/adapted to these changes. The manufacturers and suppliers adopt/embrace new technique/concept and chemistries. The industry players-PCO's, Manufacture's & Government bodies and customers expectation are crucial to maintain a sustainable yet effective termite control treatment standards

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Keywords: termite termite management, termite control, termite prevention

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ORAL PRESENTATION ABSTRACT

T1_01

STV03: A RAPID 3-HOUR TRAP FOR ADULT AEDES SURVEILLANCE

Nor Ain ATIQAH", Jara'ee ROPIAH', Rosli LINAYANTI'

Pahat District Health Office Batu Pahat Johor, Malay "Johor State Health Department, Johor Bahru, Johor, Malaysia

ABSTRACT

Presectionate e Conventional trapping methods used for Aedes surveillance often require prolonged deployment to obtain adult mosquito samples, thereby limiting their suitability for rapid surveillance and timely vector control responses. This study developed and evaluated Sticky Trap Version 3 (STV03) as a rapid tool for adult Aedes mosquito surveillance. A comparative field study was conducted using STV03, a non-attractant sticky trap used as a control, and a conventional sticky ovitrap. STV03 was developed by integrating multiple host-seeking cues, namely a dark visual surface, heat generated from hot water, and human-derived odour from worn nylon material. Two study sites with different mosquito density levels (high density: >2 mosquitoes per minute; low density: 2 mosquitoes per minute) were selected within a locality with a history of dengue outbreaks. Traps were deployed for three hours (1700-2000 h), and the study was replicated three times at each site. Captured mosquitoes were morphologically identified to the species level STV03 consistently demonstrated superior trapping performance compared with both the control and the sticky ovitrap. In high-density settings. STV03 captured 6-48 adult Aedes mosquitoes per deployment, whereas the control and the sticky ovitrap captured 0-7 and 0-1 mosquitoes. respectively. In low-density settings, STV03 captured 1-6 mosquitoes per deployment, while no mosquitoes were collected either using either the control or the sticky ovitrap. Both Aedes angypt and Aedes albopictus were successfully collected using STV03. This rapid sampling capability facilitates same-day screening for virus detection in mosquitoes, enabling the earlier detection of infected Aedes mosquitoes and supporting timely vector control interventions

Keywords: Anderellance, dengue vector mosquito trap, rapid trapping, STV3

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

ORAL PRESENTATION ABSTRACT

T1.02

OPTIMISING THE REARING OF WOLBACHIA- INFECTED AEDES AEGYPTI: FROM CHALLENGES TO SUCCESSFUL MASS PRODUCTION

Rafidah ALI, Hanipah SHAHAR, Manimegalai V., Rahman MUKHTAR', M. Helmi Ab HAMID'

Entomology and Pest Branch Kuala Langer & Pa Federal Temitory State Hou Kala Lopar Malaysi

Tropical ectious Disease Research & Education Centre (TIDREC High Impact Research Building PR Universis Malaye Kala Lumpur Malesia Enmental Heath Division City Service Department Perbadanan Pajak P

ABSTRACT

The deployment of Wolbachia-infected Aedes aegyptiis a promising strategy for reducing dengue cases in Malaysia. Therefore, large scale rearing of Wolbachile-infected Ae. aegypt is crucial for operational success. This study aimed to describe the evidence-based challenges encountered during the rearing process of Wolbachia-infected A. aegypt and to provide useful strategies that support successful adult emergence. A total of 150.000 eggs were hatched every weekly over a 14-week period, from 30 September 2025 to 3 Jan 2025 at Putrajaya Insectarium Larvae were reared in plastic trays and fed three smes prior to pupation. Temperature (27 +1°C) and humidity (70±5%) were monitored throughout the rearing process. Adults were offered a sugar solution upon emerging until the releasing day. Rearing activities were successful across all 14 cycles, achieving a mean adult emergence rate of 8164 15.66% and low larval mortality of 19 10.66 individuals. This indicates that most larvae successfully completed their development from larval to adult stages, under the applied rearing conditions. Although the overall emergence rate was satisfactory, the relatively high standard deviation suggests variability between the rearing cycles. Successful production of Walbachia-infected Ae. aegypti reflects the progressive optimisation process, whereby challenges related to larval density, feeding practices, water quality, and pupation conditions were identified and addressed over time. Ultimately, the emergence rate remained within the range reported for laboratory-reared Ae. aegypt colonies. demonstrating that targeted interventions were effective in optimising rearing performance and enhancing adult emergence.

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Keywords: Hobach, rearing Aedes aegypti dengue insectarium

ORAL PRESENTATION ABSTRACT

ZERO-FLY STRATEGY IN ENTOMOLOGICAL RISK MANAGEMENT FOR VVIP OFFICIAL EVENT OPERATIONS

Ahmad @ Abu ZURAIDAH

Entomology & Pest Unit Barang Pasang District Heath Of zaidahabuggy

ABSTRACT

T1_03

The execution of official Very Very Important Person (VIP) events demands absolute compliance with environmental health standards to prevent food poisoning. Flies are primary mechanical vectors for foodborne pathogens such as Salmonella and Escherichia coli. In the Batang Padang and Muallim districts, organising events frequently poses a high risk of severe fly infestation due to the proximity of venues to high-risk areas like poultry farms, public markets, and unmanaged roll-on/roll-off (RCRO) trash bins. This study presents the "Zero-Fly Strategy." designed to guarantee food safety and guest comfort during VVIP functions. An Entomological Risk Investigation (E) was conducted two weeks prior as part of a proactive risk management strategy to ensure a pest-free event environment. Pre-event, six core strategies were deployed: 1) Preliminary Risk Assessment, 2) Hotspot Mapping (7 km radius), 3) Risk-Based Approach, 4) Integrated Pest Management (PM), 5) Buffer Zone Strategy, and 6) Strategic Agency Collaboration Networks. During the event, biopesticide control applications were utilised, and fly density was systematically measured using the Scudder Grill Method. Cross-year operational data (January 2024-April 2020) recorded a critical initial fly index (up to 20.0) at outdoor observation stations, driven by waste management and leachate issues. However, following targeted interventions, thousands of flies (up to 4,750) were successfully trapped and eradicated within the perimeter outside the tents. This dramatically reduced the Current Fly Index within the VVIP dining zone to a completely fly-free level (0.0] for every supervised event. The success of this significant index reduction proves the effectiveness of implementing off-site interventions to prevent flies from inftrating VVIP tents. Integrated strategic collaboration involving local authorities (PBT) district health offices (PKD), district offices, the Department of Veterinary Services, and event organisers facilitated seamless sanitation operations. This systematic approach successfully achieved the objective of zero fly nuisance, thereby safeguarding public health and preventing potential disease outbreaks.

Keywords: zero-ly strategy integrated pest management PMO entomological ris investigation (E), VVIP

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

ORAL PRESENTATION ABSTRACT

INTEGRATING MOLECULAR ECOLOGY FOR SUSTAINABLE INSECT PEST MANAGEMENT

Abdul Hafiz AB MAJID

T1_04

Household & Structural Chan Entomology Laboratory School of Biological Sciences, Utah Sains Malaysia, Panang, Malayala

Oral Presection Absher-native act

ABSTRACT

The increasing complexity of urten ecosystems, coupled with rapid urbanization, climate change. global human mobility and insecticide resistance, has intensified the challenges associated with insect pest management. Sustainable solutions require a deeper understanding of pest ecology and the adoption of innovative technologies that move beyond comventional chemical control. This keynote examines how the integration of molecular ecology and urban entomology innovations is reshaping the future of sustainable insect pest management. Molecular ecology provides critical insights into species identification, population structure, dispersal dynamics, genetic diversity, and resistance evolution of urban insect pests. Through the application of DNA barcoding. population genetics, genomic tools, and microbiome analyses, researchers can uncover hidden ecological process that influence the spread and persistence of pests such as bed bugs, termites, cockroaches, and ants within urban environments. These molecular approaches enable accurate source tracking, colony delineation, and evidence-based management decisions that improve control efficacy while minimizing environmental impacts. Complementing these advances are emerging urban entomology innovations, including smart monitoring systems, Internet of Things (IoT)-based sersions, artificial intelligence-assisted surveillance, predictive analytics, and microbiome-targeted interventions. The integration of molecular diagnostics with real-time monitoring technologies facilitates early detection, precision targeting, and adaptive management strategies that reduce pesticide relance and support long-term urban sustainability. By bridging molecular science, ecological understanding, and technological innovation, a new paradigm of precision and sustainable pest management is emerging. This multidisciplinary framework enhances public health protection, safeguards urban infrastructure, and contributes to resilient cities aligned with global sustainability objectives. Ultimately, the integration of molecular ecology and urban entomology innovations offers transformative opportunities for addressing current and future insect pest challenges in an increasingly urbanized world.

Keywords: molecular ecology; urban entomology sustainable pest management, precision pest management, smart pest monitoring

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

ORAL PRESENTATION ABSTRACT

T1_05

EVALUATION OF TWO PROBE-BASED qPCR MASTER MIXES PERFORMANCE FOR FILARIAL DNA DETECTION IN MOSQUITO VECTORS

Masse RS", Tupang L², Jaafar AJ, Abdul Rahman NA', Jeyaprakasam NK, Mohd Ali MR

Wedical Entonalagy Unit fectious Disease Research Centre Insite for Medical Research (National Institutes of Heath (NH) Ministry of HeaMalesia

Entomology & Pest Brunch Sabah Health Department Kota Kinabalu Sabb

Center for Toxicology & Health Risk Studies (CORE) Faculty of Health Sciences Univers "Bactesy Unifectious Disease Research Centre, sue for Medical Research (WR) National of Heath H Mistry of Health Malaysia

[email protected]

ABSTRACT

Lymphatic filariasis (LF) is a mosquito-bome neglected tropical disease caused by Wuchereria bancroft, Brugia malay, and Brugia Timor and remains a significant public health burden in andemic tropical and subtropical regions. Molecular xanomonitoring using real-time PCR is a seraitive approach for detecting filarial DNA in mosquito vectors. This study evaluated the performance of two probe-based oPCR master mixes, TaqMan Fast Advanced Master Mix and SemiFAST™ Probe No-ROX Kit, for filarial detection in mosquito vectors. An initial comparison was conducted using four selected mosquito DNA samples collected from a filariasis-endemic area in Beaufort, Sabah. Performance was assessed based on threshold cycle (Ct) values and cost per reaction. Under the tested conditions, the SensiFAST Probe No ROK Kit produced lower Ct values than the TaqMan Fast Advanced Master Mix and showed better cost effectiveness. Based on these results, the SeraiFAST Probe No ROX Kit was used to screen 71 pooled field- collected mosquito samples from the same locality for filarial DNA detection targeting the 285 LSU rRNA gene. A total of 17 samples were positive for filarial DNA and were further analysed using a species-specific qPCR assay targeting the Hhal gene of Brugia malayi/Brugia timari Of these, four samples were confirmed positive, with infections detected in Mansonia uniformis (m3) and Mansonia bonneas (nm1). These findings suggest that the SansFAST Probe No- ROX Kit is a practical and cost-effective option for molecular xenomonitoring of LF vectors in andemic settings. Further validation with larger sample sizes and additional assay performance evaluations is needed before broader implementation.

Keywords: lymphatic filariasis, molecular sanoentoring real-time PCR Brugia meleyi Brupie timori Marsonia

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST OSCEP) 2005

ORAL PRESENTATION ABSTRACT

T1.06

EFFICACY OF METHOPRENE AND PYRIPROXYFEN ON GLYPHOSATE-EXPOSED AEDES LARVAL

POPULATIONS

Asri LAILATUL-NADHIRAH, Mohamad Johari IBAHIM, Ab Hamid NURULHUSNA, Koon Weng LAU", Chee Dhang CHEN, Saffiee NUR- SAFWANAH, Van Lun LOW, Siti Nursheena MOHD ZAIN', Othman WAN- NORAFIKAH

Faculty of Medicine, Unit Teknologi MARA (UTM) Selangor Branch, Sungai Buloh Campur Sanga Selangor Malaya Well Unit Takeolog MARA (LTM Shah Alam, Selangor, Malayala Medical Entomology List, Institute for Medical Reach MR National institute of Heath Sata Alam Shab Alam, Selangor, Malayala Center for Taxicology & Health A Studies (CORE Faculty of Health Scienc Ukara Kebangsaan Malaysia, Nala Lumpur Campus, Kuala Larpur, Malaysia Entoesofia Research Laboratory Research Facilities Cara, Department of Research Development, Liniversiti Malaya, Am Malaysia Faculty of Applied Sciences, Uniwa Tanalog MARA (LTM Pabang Branch, Jengka Campus Bandar Tun Abdul Razak Jangka Pabang Malaysia ghar hutution Centre of Excellence (Cat Tropical Infectious Disease Research and Education Canba (70REC) Universiti Malaya, Kala Lu of Diological Sciences, Faculty of Science, Unit M

Oral Presection Absheronate actr

ABSTRACT

The extensive use of agricultural herbicides like glyphosate may influence mosquito susceptibility to public health insecticides. Juvenile hormone mimics, such as methoprene and pyripraxyfer, are insect growth regulators (GRs) capable of inhibiting mosquito development and emergence. This research assessed the efficacy of methoprene and pyripraeylan, an Aedes aegypti and Aedes albopictus reference (REF) and glyphosate-exposed (GYE) larval populations. Initial bioassays on third-instar larvae of Ae. aegypt and Ae. albopictus REF and GYE populations at various concentrations determined the 50% emergence inhibition (IE) values for methoprene and pyripraryfen. First and fifth generations were subsequently exposed to these values to ascertain their emergence inhibition percentage (%). For methoprene, the IE% of Ae. aegypti REF larvae was 98.00-99.00% at an IE of 3.614 mg/L. whereas GYE populations showed an IE% of 92.00-96.00% at an IE,, of 3.828 mg/L. For Ae. albopictus larvae, an IE% of 94.00-98.00% and 8700-93.00% was achieved for REF and GYE populations at an IE of 0.182 mg/L and 0391 mg/L, respectively. Meanwhile, for pyripraxyfen, the E% of both A. aegypti REF and GYE populations at an IE of 2.000 mg/L and 0.428 mg/L, respectively, reached 99.00-100.00% As for Ae. albopictus, pyriproxylen exposure at an IE of 7731 mg/L and 0.480 mg/L resulted in an IE% of 92.00-98.00% and 99.00 % -100.00% for REF and GYE populations, respectively. Ultimately, pre-exposure to agricultural glyphosate did not reduce the effectiveness of methoprene and pyripraxyfen indicating their potential as alternative dengue vector control agents.

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Keywords: methoprene, gripen edes og Andes albopiens, insect growth regulators

ORAL PRESENTATION ABSTRACT

PRELIMINARY EVALUATION OF A LOW-COST CERAMIC-BASED ARTIFICIAL BLOOD FEEDING SYSTEM FOR AEDES AEGYPTI COLONISATION IN INSECTICIDE RESISTANCE TESTING

T1_07

Manimegalai V', Rafidah ALI*, Hanipah SHAHAR', Aishah Hani AZIL'

Entomology and Pest Branch Kemp &

Puhajaya Federal Territory State Health Office Kale Lampu Malaysia Tripical ectious Disease Research & Education Centre (TIDRECL

High Impact Research Building HR Universi Malaya Kaala Lumpur Malaysia Deputent of Parslogy and Medical Enology Faculty of Medicine, Universi Kebangsa Mala Chess Kala Lampu Malaysi

ABSTRACT

Artificial blood feeding (ABF) systems are important for maintaining mosquito colonies for insecticide resistance research. However, the Kuala Lumpur Entomology Laboratory lacks facilities for live-animal feeding, and commercial devices are expensive. This study aimed to develop and conduct a preliminary evaluation of a low-cost, ceramic-based ABF system for Aedes aegypti colonization. Aedes aegypt eggs collected from ovitraps in Wangsa Keramat were reared under standard insectary conditions. A total of 100 ten-day-old female mosquitoes were used in six blood-feeding trials with bovine blood. A ceramic pot was covered with a Parafilm membrane, which served as the artificial feeder, and tested with 6 heating methods to determine the optimal feeding temperature. The ceramic pot was heated using either a microwave (39°C-88°C) or a heating plate (38C-76°C) and then exposed to mosquitoes for one hour. Three trials failed due to temperatures that were either too low or too high, causing poor feeding or membrane damage. Successful feeding was achieved when the pot was cooled to approximately 35°C before use. The mean feeding success rate was 68%, with rates exceeding 80% in the final two trials. The ceramic feeder was approximately 113 times cheaper than a commercial feeder representing a 99% cost savings. The ceramic pot provided adequate heat retention while maintaining membrane integrity. These findings suggest that a simple, locally available ceramic pot is a cost-effective alternative for mosquito colonization in resource-limited laboratories. However, further studies are needed to evaluate long-term colony performance and reproductive outcomes across multiple generations to ensure proper insecticide resistance testing.

Keywords: artificial blood feeding low cost, colonization, Adesagt, insecticide resistance, comic pot

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

ORAL PRESENTATION ABSTRACT

T1_08

METHODOLOGY IN DEVELOPING A NOVEL INTEGRATED MALARIOGENIC POTENTIAL INDEX (IMPI) FOR MEASURING THE RISK OF PLASMODIUM KNOWLESI MALARIA TRANSMISSION

Oral Presection Absher Jonoatore in actor M

Tanrang YUSIN", Jenarun J, Syed Sharizman SAR'

Entorslag & Pest Sector Sabah State Health Office S

Faculty of Medicine & Health Sciences, Universi Malaysia Sabab (UMS) Malaysia "[email protected]

ABSTRACT

Plasmodium knowlesi malaria has become the predominant cause of malaria in Malaysia despite the successful elimination of indigenous human malaria. Existing surveillance systema primarily rely on disease incidence and entomological indicators, which do not adequately capture the multifactorial nature of zoonotic malaria transmission. There is lack of a standardized metric that integrates vector reservoir host, human, environmental and health system determinants that limits the ability to identify high-risk areas and prioritize interventions. This study proposes the development of a novel Integrated Malaricganic Potential Indes (MP) to provide a comprehensive and standardized approach for assessing the transmission risk of P. knowlesi malaria. The IMPI is developed using a sequential mixed-methods approach. The development process comprises four methodological stages: (i) conceptualization of malariogenic potential through an extensive literature review and expert consultation (i) identification of indicators using the Nominal Group Technique (NGT); (i) development of standardized scoring of indicators using the Analytic Hierarchy Process (AHP); and (v) integration of weighted indicators into a composite index using a multi-criteria weighted additive model. The proposed framework incorporates five principal domains: Vector Receptivity Host Reservoir Potensial Human Exposure Vulnerability Environmental and Ecological Factors, and Health System Performance. The study will produce a scientifically robust and reproducible Integrated Malariaganic Potential Index capable of quantifying transmission potential across endemic settings. The IMPI is designed to stratify localities according to transmission risk, facilitate spatial risk assessment, and provide a standardized metric for monitoring and comparing malariogenic potential over time. The proposed IMPI represents an innovative methodological framework for measuring zoonotic malaria transmission risk by integrating multiple determinants into a single composite metric. The index has the potential to strengthen evidence-based surveillance, improve prioritization of high-risk localities, optimize resource allocation, and support strategic planning for the prevention and control of Plasmodium knowlesi malaria in endemic regions

Keywords: Pladur knowles, integrated malariogenic potential indes (MP), malariogenic potential transirik, nominal group technique, analytic hierarchy process, aoonotic malaria

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

ORAL PRESENTATION ABSTRACT

T1_09

IPPM-R: A SMART RODENT MONITORING AND MANAGEMENT SYSTEM USING NFC, QR CODE, AND IOT TECHNOLOGIES

Mohd Kamir YUSOE", Wan Mohd Amir WAN FAZAMIN', Wan Aezwani WAN ABU BAKAR, Mustafa MAN', Harry Cheah Hoo KIET', Mastura ROSLAN

Faculty of informatics and Computing Faculty of informatics and Computing Univer Zainal Abidin Best Tengganu Malaya

'Faculty of Computer Science and Mathematics, Universiti Malayala Terengganu Kala Nera, Tangan

Malayala

ABSTRACT

Rodent infestations pose significant risks to public health, food safety, and property management, particularly in urban and commercial environments. Conventional rodent monitoring practices rely heavily on manual inspections, resulting in delayed detection, increased operational costs, and limited visibility of rodent activities. This paper presents the development of the Intelligent Pest and Rodent Monitoring System (PPM-RL a amart monitoring platform that integrates Near Field Communication (NFC), Quick Response (QR) code technologies, and Internet of Things (IoT)-based sensing for efficient rodent management. The proposed system consists of a Flutter-based mobile application, cloud-based database. web dashboard, and an ESP32-enabled smart bait station equipped with infrared (IR) break- beam sensors for detecting rodent activity in real time. Field technicians utilize NFC and QR technologies for station identification, inspection recording, and reporting, while the loT module continuously captures rodent activity events and transmits data to a centralized cloud server. The collected information is visualized through an integrated dashboard that supports activity monitoring, inspection management, and operational decision-making. The proposed architecture enhances inspection efficiency, improves monitoring accuracy, and enables near real-time visibility of rodent activities compared to traditional manual approaches. The system establishes a foundation for future integration of artificial intelligence (AI) and predictive analytics for intelligent pest management applications.

Keywords: rodent menting Intemet of Things (T), NFC, QR Code, ESP32, smart but station, pest management, mobile application, real-time monitoring

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

ORAL PRESENTATION ABSTRACT

T2_01

STRENGTHENING BORDER HEALTH SECURITY THROUGH INTEGRATED VECTOR SURVEILLANCE: EVIDENCE FROM PENGKALAN KUBOR INTERNATIONAL POINT OF ENTRY, MALAYSIA (2023-2025)

Md Yunus SYARIFAH NADWAH, Hassan HASNEEZAH, Djoharnis JUNAIDI', Harun MOHAMAD HAFIZ, Mohd Wajiah MOHD KHAIRUL HANAN', Mohd Nordin NUR AKMARINA, Mohd Jaafar NORJAIZA²

Ocal Presentation-actor Sale Sho

Tampa District Health Office Tessa Kelan Kelas State Health Department, Kota Bhanu K

"[email protected]

ABSTRACT

Vector-borne diseases remain a significant public health concern at international points of entry (PoE) due to the potential introduction and cross-border spread of disease vectors and reservoirs. In accordance with the International Health Regulations (HR 2005), entomological risk assessments (ERAs) are conducted to support surveillance and risk assessment activities within PoEs and the surrounding areas. This study presents findings from three years of annual ERAS conducted at the Pengkalan Kubar International Point of Entry, Kelantan, Malaysia, between 2023 and 2025. The assessments included larval surveys, ovitrap monitoring and rodent trapping. Larval surveys were conducted to determine the Aedes Index (A) Breteau Index (B), and Container Index (C), while captured rodents were identified to the species level and examined for flea infestation. A total of 34 positive Aedes breeding sites were identified. comprising Aedes albopicts (76.5%). Aedes aegypti (20.0%), and mixed breeding species (2.9%). The principal breeding habitats were discarded containers and water storage containers. The annual AL, B, and Cl values were 3214, 64.0, and 13.63 in 2023: 8.82, 150, and 3.63 in 2024 and 33.33, 410, and 12.04 in 2025, respectively. Ten rodents were captured, predominantly Rat ratus (60.0%). No flea infestation was detected (Flea Index=0). The predominance of Aedes albopictus and the persistence of breeding habitats in artificial containers indicate a continued receptivity to Aedes-borne diseases. The absence of fea infestations indicates a negligible risk of plague transmission. Sustained surveillance and targeted vector control interventions remain essential to strengthen border health security and support compliance with IHR (2005).

Keywords: Aedes albopictus Aedes aegypti entomological risk assessment (ERAS) peint of entry (PE) survelance, Kelentas

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

ORAL PRESENTATION ABSTRACT

T2.02

MOLECULAR CHARACTERISATION OF THE BLOOD FEEDING SOURCE OF ANOPHELES MACULATUS IN A PLASMODIUM KNOWLESI TRANSMISSION AREA

Abdul Jabir JAAFAR, Mohd Nazmi MD NADZRI, Ainul Huda KHAIRUL AZMAN', Nurul Asmaa ABDUL RAHMAN', Rezki Sabrina MASSE

Wedical Entomology Chic infectious Research Care, lestitute for Medical

National sites of Health DH Shah Ales Malaysia

Biomedical Epidemiology Unit Special Resource Cene stitute for Medical Research onl estates of Health (MH) Shah Alam Mys

ABSTRACT

Ocal Presentation-to

Malaysia has eliminated indigenous human malaria, however, zoonotic malaria caused by Plasmodium knowles/ remains a major obstacle to WHO malaria elimination certification Although Anopheles maculatus is not traditionally recognised as a primary zoonotic malaria vector its widespread distribution in knowlesi-endemic areas may suggest potential epidemiological importance. A recent study in Indonesia had incriminated An maculatus as a potential vector of zoonotic malaria. Hence, this study investigated the blood-feeding patterns of An maculans collected from a P. knowlesi transmission area in Negeri Sembilan, Malaysia, Adult female An maculatus were collected using the human landing catch (HLC) method in Mukim Pantai, Negeri Sembilan, from March to June 2024. A total of 150 morphologically identified, non-engorged females underwent molecular blood meal analysis to detect traces of host DNA from their previous blood meal. Genomic DNA was extracted from dissected abdomens using the DNeasy Tissue KR (Qiagen, Germany) Vertebrate host DNA were detected via nested polymerase chain reaction (PCR) targeting the mitochondrial cytochrome c oxidase subunit I (COI) gene, followed by species-specific PCR assays for human and monkey DNA. Of the 150 samples analysed. 70 (48.7%) tested positive for vertebrate DNA, All vertebrate-positive samples contained human-derived blood meals, while no mankey DNA was detected. These findings confirm human-feeding activity by An maculatus in the study area, providing baseline evidence of its anthropophilic feeding behaviour. Further studies across broader geographical and ecological settings are recommended to better characterise the blood-feeding patterns of An. maculatus and its epidemiological significance in zoonotic malaria transmission.

Keywords Zoonotic malaria, Anopheles ca Pladiaren host preference, Col gene

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

ORAL PRESENTATION ABSTRACT

T2.03

COMMUNITY DIVERSITY AND PREDICTIVE DISTRIBUTION OF JAPANESE ENCEPHALITIS VIRUS VECTORS ACROSS SABAH LAND TYPES

Ag Shazmeer AG SAFREE', Khairul Azman KARIM, Chian HO², Adlar Ryan NGIAM², Ping-Chin LEE", Sylvia DAIM', Song-Quan ONG

Institute for Tropical Biology and Conservation Universi Malaysia Sabah Kora Kinaba Faculty of Science and Technology Universi Malaysia Sabah Kota Kinabah Sabuk Malaysia Faculty of Medicine and Health Sciences Universi Malaysia Sabah Kota Kinabalu Sabat Malaysia "[email protected]

Ocal Presentation-actor Suva Sh

ABSTRACT

Japanese encephalitis (E) remains a critical yet neglected mosquito-bore viral threat in Southeast Asia. Effective surveillance is hindered by insufficient knowledge of the distribution of Culex mosquitoes, particularly in regions such as Sabah, where rapid land-use changes and diverse topography create complex ecological niches. This study aimed to characterise the Culex mosquito community across multiple districts and map their spatial distribution in Sabah Mosquito data were collected using human-baited double nets, CDC light traps, and BG- Sentinel (BGS) traps across four major divisions: Tawau, Semporna, Sandakan, and Kudat during the peak biting period. To assess community differences, Culex diversity was calculated, and differences among districts were evaluated using ANOVA. Detrended Correspondence Analysis (DCA) was used to examine species composition across different habitats, and machine leaming models were constructed and compared for Species Distribution Modelling (SDM) to study their spatial distribution. A total of 3367 mosquitoes were collected and identified, with the secondary vectors Cx. quinquefasciatus (55%) and Cs. vishnui (42%) emerging as the dominant species. DCA ordination revealed clear niche differentiation: Cx. vishnui was predominantly associated with coastal habitats, while the primary vectoc Cx. tritaeniorhynchus, was more commonly found in inland forested environments. SDM projections identified highly suitable areas for Cx tritaeniorhynchus along the western coastline and northern Kudat, while the distribution of Cx. quinquefasciatus closely matched areas of high human population density in western Sabah. These spatial distribution maps provide a valuable framework for targeted vector control, particularly at the interface of agricultural and peri-urban high-risk zones.

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Keywords: Culex, random forest, community ecology Japanese Encephalitis, vector risk mapping

ORAL PRESENTATION ABSTRACT

T2_04

MOLECULAR EVIDENCE OF ORIENTIA TSUTSUGAMUSHI CIRCULATION IN CHIGGERS AND SMALL MAMMALS FROM HUMAN SCRUB TYPHUS HOTSPOTS IN PAHANG, MALAYSIA

Ernieenor Faraliana CHE_LAH", Wan Norqariatul Akidah WAN ABDULLAH², Nurhanani IBRAHIM', Ariana Ace JOHN', Ellyncia BD- BILIN', Muhamad Al 'Azim BAHARUDIN', Yoggendran INDIRAN

Worsley Unit, defectious Diseases Research Cente, estate for Medical Research Ministry of Health Malaysia National lestitute of Houth Complex, Setia Alam Shah Alam Selangor M Entosalogy & Pest Unit Patang State Health Department Lorong

Bandar Indera Mahon Kastan Panang Malaysia

Bentong District Heath Offee Bentong Pahang Malaysia as Dishict Houth Office Kas

"Enterology & Pest Unit Terserion District Health Office Terrion Pahang, Malay

• "[email protected]
• ABSTRACT
• Pang Masa

Ol Presentation-ector Suvale Shoes

Scrub typhus (ST) is a neglected vector-borne zoonosis caused by Orientati and transmitted to human through the bite of infected chiggers. In Malaysia, the increasing number of human cases has highlighted the need to better understand the ecological factors driving ST transmission. Following reports of more than 60 laboratory-confirmed ST cases in Pahang during 2024, an investigation was conducted to identify potential vector and animal hosts associated with transmission in selected case localities. Small mammals were trapped in suspected infection hotspots, including residential surroundings and workplaces of confirmed cases. Captured animals were examined for chigger infestation, and collected chiggers were identified morphologically. A total of 95 small mammals were captured, with 62 (95.3%) were infested with chiggers, yielding a chigger index of 40.3. Overall, 3,831 chiggers were recovered. comprising predominantly Leptotrombidiure dalianse (46.7%), followed by Ascaschoengusti indice (33.9%) Leptotrambidiuer spp. (18.4%), and Helenicula mutabils (10%) DNA extracted from 188 pooled of chiggers, blood and spleen fissues was screened for Q. tagasi using nested PCR targeting the 66-kDa TSA gene. The pathogen was detected in 13 (6.9%) chigger pools, all identified as L. delense, as well as in 11 spleen and six blood samples. Sequence and phylogenetic analyses revealed high nucleotide similarity (87-100%), with the Karp genotype predominating, followed by Gilliam, Kato, and TA763 genotypes. The detection of august in both vectors and small mammals provides evidence of pathogen circulation in Pahang, underscoring the importance of continued surveillance and ecological studies to support ST prevention and control

Keywords: scrub typhus chiggers, Orientousgameshi rodents, Malaysia

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCP) 2005

ORAL PRESENTATION ABSTRACT

T2.05

WINGED GUESTS IN THE WARD: RECURRING DIPTERAN INFESTATIONS IN A PUBLIC HOSPITAL INTENSIVE CARE UNIT

Ocal Presentation- cty Survace Sho

Kalaivaani ADAKALAM, Gurpreet Kaur KARPAL SINGH', Khadijah KHAIRUDDIN'

Kang District Health Office Klang, Selangor Ma Selangor State Health Department Kang Selangor, Malaysi

"[email protected]

ABSTRACT

Recurrent insect infestations in critical healthcare settings pase severe hygiene and myiasis risks. This case study investigates repeated dipteran nuisances in an intensive care unit (ICU) of a public hospital in the Klang Valley from September 2024 to October 2025. An entomological investigation was conducted utilising sticky traps and extensive structural inspections across the ICU, specifically within the shaft rooms, tollets, and the space above celing Initial investigations identified scuttle flies (Megaselia spp). Subsequent investigation revealed a shift to predominantly drain flies (Psychoda spp.). Non-biting midges (Chironomidae) were also found. The primary breeding sources were accumulations of organic matter resulting from leaking plumbing, structurally compromised shat rooms, and inappropriate floor traps. The recurrence of the infestation was attributed to delayed structural repairs, which was further compounded by ineffective pest control measures executed by the pest control operator (PCD) appointed by the hospital's non-clinical support services. Eradicating dipteran pests in high-risk and ultra-high-risk zones necessitates a holistic integrated pest management (IPM) approach. Effective control must prioritise source reduction through prompt structural plumbing repairs, targeted larviciding, thorough sanitation, and the engagement of certified pest management professionals.

Keywords: Intensive care unit (CU) drain fix soute long plumbing stidy baps, integrated pest management (PM)

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

ORAL PRESENTATION ABSTRACT

T2_06

RAPID DENGUE DETECTION OF TRANSOVARIAL TRANSMISSION IN AEDES VECTORS FROM CENTRAL JAVA PROVINCE, INDONESIA

Shamshul Anuar INTAN-UMAIRATH, Isa Mohamad RODI Sayono SAYONO, Mohd Shah NURUL-AZIRA", Didik SUMANTO', Othman WAN-NORAFIKAH**

Faculty of Medicine, Universi Teknologi MARATM Selangor Branch, Sungai Buon Campus Jalan H Sungai Buloh, Selangor Malaysia

HW ReNe Universis Teknologi MARA ATM Shah Alam Selang Masa Faculty of Public Healersitas Muradiyah Semarang Jalan Kadanganda Raye No Semarang Cental Java Province does

ABSTRACT

Ol Presentation-ect Suvage Sho

Dengue virus (DENV] can be transmitted through both horizontally and vertically within Aedes mosquito populations, potentially resulting in a dengue outbreak Vertical transmission specifically transovarial transmission, occurs when infected female mosquitoes transmit the virus to their offspring. This study aimed to determine the occurence of transovarial transmission of DENV among Aedes vectors collected from tourist attractions in Central Java Province, Indonesia Mosquito larvae were collected using ovitraps from seven tourist attractions, consisting of beaches, gardens, a heritage site and community parks. The collected larvae were reared under laboratory conditions and morphologically identified at the adult stage. First generation (F1) female adult mosquitoes of eight Aedes aegypti and eight Aedes albopictus populations, including laboratory strains as a reference, were grouped into pools of five individuals, yielding six pool per population. In total, 240 As. aegypti and 240 Ae. albopictus mosquitoes from 48 pools per species were subjected to rapid DENV detection using the Bioline Dengue NS1 Ag rapid test kit. All teated mosquito pools were negative for the dengue NS1 antigen (pool positivity rate=0%), indicating no detectable transovarial transmission among the tested populations. These negative findings could be attributed to low DENV transmission during the sampling period, low natural infection rates within the mosquito populations, or viral loads falling below the detection Emit of the NS1 rapid test kit. Although no positive samples were detected, the NS1 rapid test remains a beneficial tool for the field screening of dengue virus.

Keywords: Dengue NS1 antigen nopid detection, Andes angypt, Aedes albopictus Indonesia

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

ORAL PRESENTATION ABSTRACT

FIRST MALAYSIAN RECORD OF SCHOENGASTIA PSEUDOSCHUFFNERI AND BASELINE DATA FOR SCRUB TYPHUS SURVEILLANCE

T2.07

Zainal Abidin S, John AA', Ishak MS', Foo PC', Anak Mawang C', Nik Kamarudin NA', Zakaria N, Che Lah EF, Stekolnikov AA'

Worsley Unifestous Diseases Research Centre, lestitute for Medical Research Nationalistes of Health Ministry of Health Malaysia Shah Ales Selangor Malaysia "Vectorbose Disease Control Sabah State Health Department Ministry of Health Malaysia Kota Kinab Sabuk Malasia

Zoological India Russian Academy of Sciences Salt Petersburg Aus

Ocal Presentation- cty Suva Sh

ABSTRACT

Chigger mites are important vectors of scrub typhus and their common hosts are small mammals. Although no confirmed cases have been reported in Beaufort, Sabah, a previously suspected case highlights the need for continued vector surveillance. The district's mosaic of palm oil plantations and coastal village scrub-farms creates a high-contact interface prime for zoonotic disease spillover. This study investigated chigger infestations among small mammals inhabiting agricultural and coastal environments in Beaufort, Sabah. Small mammals were trapped across sites representing distinct landscape matrices and examined for chigger infestation. Captured animals and collected chiggers were identified morphotaxonomically and screened for Orientia tsutsugamushi using nested PCR. A total of 37 small mammals were examined, of which eight infested, yielding an infestation prevalence of 21.6%. A total of 259 chiggers were recovered, with Sandantys mualiani harboured the highest chigger burden (n= 130), followed by Rattus rattus [n =61) and Callosciurus netas (n=42). This study documents the first formal record of Schoangasta pseudoschuhen in Malaysia, identified as the dominant chigger species across multiple hosts, alongside Aschoenguatia indica and the primary scrub typhus vector Leptotrombidium defense. Although O. tsutsugamushi was not detected in chigger pools or host tissues, the discovery of Schoengastia pseudoschuffneri significantly expands current knowledge of chigger fauna in Beaufort, Sabah. These findings provide baseline data anchigger-host associations in coastal and agricultural environments, and support continued surveillance to better understand scrub typhus ecology in this region.

Keywords: chiggers Trombiculidae Scheengasta podeschuhe vector surveillance subtypes, Sabah

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

ORAL PRESENTATION ABSTRACT

T3.01

EVALUATION OF VECTOBACⓇ WG, A WATER- DISPERSIBLE BACTERIAL LARVICIDE FORMULATION, FOR THE CONTROL OF ANOPHELINE AND CULICINE (ORDER: DIPTERA) MOSQUITO VECTORS IN SARAWAK, MALAYSIA

Rezal BOHARI, Siti Fatimah SAMSUDIN, Richard NYALAU², Tze Yong LOO³, Micheal PELITINI", Teh Jo HUN'

Sibu Divisional Health Ofice. S

Ser

Kant Divisional Health Oe, Kant Sarawak Malaysia

'Dept of Cnity Medicine and Public Health Facolty of Medicine and Health Sciences, Universi Malaysia Sarawak Kota Samarahan Sara "[email protected]

ABSTRACT

Presentative-Scepcity and C

Larviciding is a supplementary tool for malaria control in Malaysia; however, standardised operational guidelines remain limited. This study evaluated the efficacy of VectoBac WG (B1) dosages at 500 and 1000 grams per hectare, dispersed via a STIHL SR420 mist blower, on the suppression of anopheline and culicine larvae in the Kapit district, Sarawak. A three-phase trial involving eight permanent pools was monitored weekly for four months. The pools were located within a 100-metre distance from the local community Pools used for Bti treatment in Phase 1 were used as controls in Phase 2 and were alternated again in Phase 3. Larval populations were quantified using a standard dipper across 10-15 fixed sampling spots per pool; populations were determined before treatment, as well as 24 hours and 7 days post- treatment. The study focused on the impact of Bti treatment on the L3/L4 stages, as these are more likely to develop into the next generation of adults compared to the L1/L2 stages. In Phase 1, the 500 g/ha and 1000 g/ha dosages achieved a significant 100% larval reduction within 24 hours compared to the control (p<0.05). In Phases 2 and 3, the 500 g/ha rate maintained a 98.9%-100.0% reduction. Post-treatment densities remained statistically stable after a one-week non-intervention period (pa006), with Spearman's correlation confirming that larval reduction was entirely independent of rainfall (p>0.05). Cost-effectiveness analysis identified the 500 g/ha dosage as optimal, yielding an Incremental Cost-Effectiveness Ratio (CER) of RM 9.07 for anophelines per percentage of reduction. In conclusion, an operational dosage of 500 g/ha ensures immediate, maximum larval suppression. However, its shart one- week efficacy necessitates integrated deployment within existing Malaysian malaria control strategies.

Keywords: Vector WG, larvicides, anaphalines, cucines, malaria, Sara

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCP) 2005

ORAL PRESENTATION ABSTRACT

T3.02

EFFECTIVENESS OF A COMMERCIAL ORGANOPHOSPHATE TEMEPHOS AGAINST AEDES ALBOPICTUS LARVAE FROM RESIDENTIAL SITES IN PENINSULAR MALAYSIA

Noor Izuddin NUREEN-AFRINA', Navindra Kumari PALANISAMY¹², Saffiee NUR-SAFWANAH", Asri LAILATUL-NADHIRAH', Isa Mohamad RODI, Shamshul Anuar INTAN-UMAIRATH', Nizam NABIHA", Saifulazmi NUR-FADLIN', Wan Nor Saidah AZLAN, Mohd Nasir NADZIMAH, Othman WAN-NORAFIKAH**

'Faculty of Medicine, Univar Teknologi MARA (UTM) Selangor Branch, Sungai Buloh Campur, Sungai Buloh Selangor, Malayzia

Presecta Sascity and Control Approac

HW Real Live Faculty of Applied Sciences, Uni Faculty of Pharmacy that Teknologi MARA (LATA, Shah Alam, Selangor, Malaya arst Takealogi MARA (LTM Pabang Branch, Jengka Campus Bandar Tan Abdul Razak langka, Pahang, Malayala knabg MARA ATM Selangor Bunch, Puncak Alam Campus Bandar Puncak Alam, Sala horaich@utnedumy

ABSTRACT

Despite countless control efforts by authorized health bodies and the public. dengue cases in Malaysia main alarming. Dengue vector control relies mainly on insecticides, such as organophosphates, to target mosquito larvae and adults. However, their continuous use may initiate resistance in dengue vectors, namely Aedes aegypt and Aedes albopictus. This study evaluated the effectiveness of a commercial organophosphate temephos in inducing mortality in Ae. albopictus larvae from three dengue-risk and three dengue-free residential sites in Peninsular Malaysia. Water replenishment and non-replenishment tests were conducted under laboratory conditions by exposing field populations and a laboratory reference strain of third instar Ae. albopictus larvae to temephos in plastic containers at the recommended operational dosage of 1 mg L-1 for 40 weeks. Results demonstrate that over 30.00% larval mortality was achieved during the first four weeks of exposure across all tested populations. Mortality decreased to 80.00% -90.00% between weeks five and 11, 50.00% - 60.00% between weeks 12 and 12 and below 20.00% by weeks 34 to 40. These findings indicate that temephos remains effective in exterminating Ae. albopictus larvae across all residential sites for about four to six months, even with weekly water replenishment and regardless of their prior exposure to public health insecticides Nevertheless, routine monitoring of mosquito susceptibility is important to ensure the continued relevance of insecticide use particularly temephos, while mitigating resistance risks and sustaining the effectiveness of dengue control strategies.

Keywords: Asles actus organophosphate, terephos, residential sites, water replenishment, Maleysi

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

ORAL PRESENTATION ABSTRACT

T3.03

RESISTANCE PROFILING OF AEDES AEGYPTI USING INTENSITY CONCENTRATIONS ENHANCES THE EVALUATION OF INSECTICIDE EFFECTIVENESS FOR VECTOR CONTROL

Basli ROSILAWATI, Misrawi NORLAILI, Rosli SHAZREENA', Ariffin FARAH DIANA', Kamarudin NUR AMALINA', Abdul Razak SHAIRAH', Wan KIEW-LIAN²

Medical Enterology Unit, Infectious Disease Research Centre of Health, Ministry of Heath, Shah Alam, Selangor, Malay Department of logical Sciences and Biotecheology. Facahy of Science and Technology Univers

Kabangan Malaysia, Bangi Selangor, Malaysia for Medical Research National

'Department of Applied Physics, Faculty of Science and Technology Utara Kebangsaan Malaya Selangor, Malaysia [email protected]

ABSTRACT

Presentatie-Scity and Contr

The detection of insecticide resistance is pivotal for characterising the phenotypic resistance of field- collected Aedes aegypti, facilitating early identification of resistance trends to safeguard the efficacy of chemical interventions in vector control programmes. While the World Health Organization (WHO) adult tube test is the standard screening method, its reliance on binary resistance and susceptibility data limit the ability to categorise insecticides by their degree of resistance across insecticides. To address this, a probit analysis of adult mortality of a field collected strain from discriminating and intensity bioassay, was performed. The LC values were normalised using the concentration ratio framework. The conelation between the concentration ratio data matrix and the slope of field strains across various insecticides was evaluated, enabling the inference of resistance magnitude. The results demonstrate that primiphco-methyl showed high efficacy, whereas significant intra-population variability in resistance intensity existed among pyrethroids and malathion, with lambda-cylin ohibited the highest resistance, followed by permethrin and alpha-cypermethrin Utilising mortality data derived from WHO-recommended discriminating and interaity concentrations enables a more detailed evaluation of resistance beyond the binary classification of standard susceptibility tests. Such data-driven strategies are essential to improve the interpretation of insecticide resistance profiling. They provide a clearer picture of current resistance status, ultimately allowing for more informed decision-making regarding insecticide rotation in vector control programmes.

Keywords: dengue, pynthroids, intensity resistance, WHO adult assay Aedes aegyp

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCP) 2005

ORAL PRESENTATION ABSTRACT

T3.04

INVOLVEMENT OF DETOXIFICATION ENZYMES IN INSECTICIDE RESISTANCE AMONG AEDES ALBOPICTUS FROM AGRONOMICAL AREAS OF WEST MALAYSIA

Othman WAN-NORAFIKAH, Chee Dhang CHEN', Mohd SOFIAN-AZIRUN

Faculty of Medicine, UniveTakologi MARA (LT) Selangor Branch, Sungai Buloh Campur Sungai Bul Selangor, Malaya

"HW Real Live Teknologi MARA (UTM Shah Alam, Selangor Malaya

Travel Research Laboratory; Research Facilities Centre Department of Research Development, Uni Malaya, Kuala Lumpur, Malaya

Sa Presectability and Con tute of Biological Sciences, Faculty of Science, Univ horafiah@utmedumy

ABSTRACT

Malayalam

The extensive use of insecticides to control mosquito vectors can lead to the development of insecticide resistance, with increased metabolic enzyme activity serving as a primary mechanism. This study ascertained the involvement of detoxification enzymes in insecticide resistance among Aedes albopictus from agronomical areas in West Malaysia. Enzyme microassays for non-specific esterases (EST), mixed function oxidases (MFO), glutathione- S-transferases (GST), and insensitive acetylcholinesterase (AChE) were conducted on Ae. albopictus larvae and adults. Significantly elevated a-EST activity was exhibited in As. albopictus adults from all three types of agronomical areas, while significantly elevated a-EST activity was observed only in Ae. albopictus adults from rice cultivation areas and rubber estates. Elevations in larval a-EST and a-EST activities, alongside MFO activity in both larvae and adults across all area types, were statistically insignificant. Significantly elevated GST activity at the larval stage was discovered in Ae. albopictus populations from bath rice cultivation areas and rubber estates; however, at the adult stage, this elevation was only demonstrated in Ae. albopictus from rice cultivation areas. Furthermore, findings from the AChE enzyme microassay showed the effectiveness of proposar as an adulticide across all agronomical areas, though it was only effective in controlling Ae. albopictus larvae within oil palm plantations. In brief, the involvement of each detoxification enzyme in insecticide resistance pathways across different life stages of Ae. albopictus was autonomous and varied. Further studies are required to broaden our understanding of the roles of detoxification enzymes in the metabolic resistance of mosquitoes.

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Keywords: Aedes albopictus, insecticide resistance, detoxification enzymes, Malaysia

ORAL PRESENTATION ABSTRACT

T3.05

ASSESSMENT OF FOGGING MACHINE SPRAY DISTANCE AND ITS EFFECTS ON VEHICLE SURFACES

Ariffin FD, Kamarudin NA', Abdul Hadi A', Muhamed KA', Tan BCH', Hairuddin LH', M. Jaafar NF', Khairuddin K², Rasli R'

Medical Entornology Unit for Medical Research Nationales of Health, Ministry of Healt Malayala

Jabatan Kata Negeri Selangor, Malayala

Tahdone a@mohgowy

ABSTRACT

Thermal fogging is a common method for controlling adult mosquitoes in public health programa Although thermal fogging is an established method for large scale vector management, its effects on adjacent surfaces have not been systematically evaluated. This study investigated how nozzle distance and exposure duration affect white automotive paint, providing experimental data to optimize safety protocols and evidence-based guidance for public health thermal fogging. A field simulation using a handheld thermal fogging machine. the Agralog AF-35, was conducted. The experiment compared two diluents, water and diesel, in combination with an adulticide at different distances from the application site: far distances of 1 m and 3 m, and close distances of 5 cm and 10-15 cm. For close-distance applications, an infrared thermometer measured the nozzle temperature, and the time until visible discoloration was recorded. Visible changes were scored from 0 (no visible change) to 4 (severe yellowing) of the painted surface. Applications at standard distances of 1 m and 3 m resulted in a stain score of 0, with the paint remaining intact regardless of the diluent used. In contrast, close-proximity spraying caused significant surface damage. At 10-15 cm, the surface temperature reached approximately 170 °C, producing visible discoloration after 12 seconds of exposure. At 5 cm, the temperature increased to 196 "C, resulting in severe yellowing within 19 seconds. These findings indicate that surface discoloration is associated with the thermal effects of the fogging nozzle rather than the chemical composition of the insecticide formulations. Consequently, maintaining a minimum operational distance of 1 m is recommended to prevent vehicle surface damage during public health field operations. These results provide evidence-based guidelines for mosquito control personnel to minimize property damage while maintaining the effectiveness of public health interventions.

Keywords: Thermal fogging water control spray distance public health

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

ORAL PRESENTATION ABSTRACT

T3.06

EVALUATION OF INSECTICIDE RESISTANCE IN AEDES AEGYPTI FROM KUALA TERENGGANU FOR SUSTAINABLE VECTOR CONTROL

Nur Sofea Kamelia DJAFAR', Suzana MISBAH", Mohd. Khalil JUSOH"

Gal Presectation Scity and Control Approac

Faculty of Marine Science and Environment Universi M

Kuala Terengganu, Teen Mas

Faculty of Marine Science and Environment Univers Malaysia Terengga

Entomology and Pest Lenggan Teresh Malaysia scana martedams mohdi hagmahgary

ABSTRACT

Dengue fever remains a major public health concern in Malaysia. The disease is transmitted primarily by Aedes aegypt and is exacerbated by high mosquito abundance. Current vector control strategies rely heavily on insecticides; however, their effectiveness is compromised by the emergence of insecticide resistance in mosquito populations. This study aimed to assess the resistance status of Ae. aegypti obtained from PPR Hiliran, Kuala Terengganu, against pirimiphos-methyl, an organophosphate, and cyfluthrin, a pyrethroid insecticide. Before the experiments, laboratory conditions were optimised, maintaining a temperature of 26-28°C and a relative humidity of 60-80% to facilitate mosquito rearing. Eggs of Ae. aegypti were collected using avitraps and reared under these controlled insectary conditions. Emerged adults were morphologically identified and subjected to WHO-standard adult bioassays to assess resistance. The results demonstrated that optimal laboratory conditions supported the production of healthy uniform-aged adult mosquitoes suitable for bioassay testing. Resistance profiling showed that the mosquitoes were resistant to pirimiphos-methyl at 60 mg/m² (81.25% mortality) but susceptible at 120 mg/m² (38.75%), indicating concentration-dependent susceptibility. In contrast, cyfluthrin produced low mortality at both 0.15% (63.79%) and 0.3% (67.5%), confirming pyrethroid resistance according to WHO criteria, highlighting potential challenges for local vector control programmes. This study emphasises the importance of optimised rearing conditions for reliable resistance assessments and provides critical baseline data on A. aegypt insecticide resistance in Kuala Terengganu. The findings can inform evidence-based strategies for managing dengue vectors and contribute to the development of sustainable and effective vector control interventions.

Keywords: Aedes aega dengue, insecticide resistance vector contrel laboratory rearing bioassay

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

ORAL PRESENTATION ABSTRACT

T3_07

EVALUATING THE RESIDUAL EFFICACY OF DIFFERENT INSECTICIDE FORMULATIONS AGAINST MOSQUITO VECTORS

Nurulhusna AB HAMID*, Norlaili MISRAWI, Ezita Qaisara RIDZUAN, Fatin Nur Adlina ROZAIDI, Aimi Afiqah MOHAMMADIN, Muhammad Afiq Asyraf MOHD FAIZOL, Mohamad Haziq Uzair MOHD ROSLI, Mohd Farizul MOHAMMAD FATIHI, Farah Diana ARIFFIN

Medical Entoreslogy Unit defectious Diseases Research Centre, estate for Medical Research National sites of mea Ministry of Health Malesia Setia Alam Shah Alam, Selangor Malaysia

ABSTRACT

Chemical control via residual spray is a critical component in managing vector-borne diseases. While synthetic pyrethroids like deltamethrin were historically effective, escalating development of insecticide resistance seriously threatens sustained vector management. This study evaluates the residual effectiveness of different insecticides formulations with different active ingredients against diverse vector populations. Insecticide formulations were prepared according to manufacturers' specifications. Standardized WHO Wall Deposit Bioassays were conducted every 2 weeks to observe and evaluate the mortality percentages against laboratory and wild strains of Aedes spp. Culex quinquefaciatus, and Anopheles cracens Data from 4,445 valid observations were analysed using a Generalized Linear Model (GENLIN) with a binomial distribution and logit link function, incorporating two-way interaction terms to track performance changes over time. Following one spray cycle, all factors-insecticide type, mosquito strain, time, and surface height-significantly affected mortality (p < 0.001). Each incremental day post-spraying caused a significant 7.5% reduction in overall mosquito mortality odds (OR 0.925), suggesting residual degradation. The lower surface height (40 cm) resulted in 22.4% lower mortality odds compared to the 120 cm upper level (OR = 0.776). Crucially, the model proved that insecticides lose their killing power at vastly different speeds over time, with certain formulations maintaining a more stable residual presence. Relying solely on raw mortality percentages, it can hide real mosquito resistance dynamics in the field. Insecticide longevity is highly dependent on the specific chemical formulation and targeted vector strain over time. Vector control programmes must consider local insecticide resistance status and transition toward targeted chemical rotation strategies rather than a "one-size-fits- all approach

Keywords residual spray insecticides formulation residual efficacy mortality

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

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POSTER PRESENTATION ABSTRACT

P01

ASSESSING THE RELATIONSHIP BETWEEN AEDES INDICES AND BREEDING CONTAINERS IN DENGUE HOTSPOTS: EVIDENCE FROM LEMBAH PANTAI, 2023-2025

Noorazlin MOHD ALI, Rafidah ALI, Hanipah SHAHAR', Noriklil B ISMAIL

Lembu Panal District Health Office Kuala Lumpur, Malays

"sical electious Disease Research & Education Center (TIDRECL High pact Research Building (H Universi Malaya Kala Lumpur Males

Etology and Pest Branch Ke Lumpur & Putrajaya Federal Tentory State Health Office Kale Cam

*[email protected]

ABSTRACT

Deral Poster Presen

In Malaysia, dengue fever remains a significant public health concam, with Aedes mosquito serving as the primary vectors. This study aimed to assess temporal trends in Aedes density and examine the relationship between the breeding container index and mosquito abundance in dengue hotspot localities. Entomological surveillance was carried out across 23 dengue hotspot localities in the Lembah Pantai District, Kuala Lumpur, from 2023 to 2025. Three standard indices were recorded the Aedes Index (A), Container Index (C), and Premise Index (P). The relationship between container positivity and mosquito density levels was examined using Spearman's rank-order comlation. The results showed that mosquito demity peaked in 2024, with a median Al of 16.67 and alof 11.76, indicating widespread infestation across the district. By 2025, all indions had declined to near-zero levels (Al 0.07; Cl and Pl=0.06), representing a reduction of more than 99% compared with 2024 Spearman's correlation between Cl and Al increased from a moderate, non-significant association in 2023 08-0410, p=0.052] to a strong and highly significant correlation in 20240458 p<0.00 remaining significant in 2005 (r=0.833, p=000). The consistent association between Cl and Al across the study period suggests that breeding container positivity is a reliable indicator of Andes population intensity. The marked decline observed in 2025 may reflect successful vector control interventions, environmental influences, or both. These findings provide evidence-based support for utilising container-focused surveillance as a key component of dengue prevention and vector management.

Keywords: Andes Indices (A.), Premise Indices (P Container Indices (C), Aedes at Adap

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

P02

POPULATION DYNAMICS OFANOPHELES INTROLATUS AND ITS POTENTIAL ROLE IN ZOONOTIC MALARIA TRANSMISSION IN HUTAN SIMPAN SEMPAM, RAUB, PAHANG, MALAYSIA

Nurhanani IBRAHIMI", Wan Norqariatul Akidah WAN ABDULLAH², Noorlaili ISMAIL, Mohd Maher IBRAHIM, Rezki Sabrina MASSE", Azahari ABDUL HADI, Mohamad Shakirudin NGAH, Izhan Shahrin

Marof Peter Presenc

JAAFAR

Barang Death Oice Bentong Pahang May Pahang State Health Offca, Kuantan, Pahang Malaya "Lipis Dat Heath Ge, Nuala Lipis, Pabang Malaysia "natute for Medical Reach, Sata Alam, Salangor, Malaya Kata Bharu Public Health Laboratory Kota Bharu, Kalantan, Malaya

*hanenials@gm

ABSTRACT

Zoonotic malaria remains a significant public health concern in Malaysia, particularly in forested areas with active human, vector and macaque interactions. In Raub, Pahang, reported cases have increased in recent years. This study aimed to identify species within the Anopheles leucasphyrus group present in Hutan Simpan Sempam and assess their potential role in zoonotic malaria transmission. Mosquito sampling was conducted monthly from March to December 2025 using the outdoor human landing catch (HLC) method. The collected mosquitoes were morphologically identified and screened for Masmodian parasites using polymerase chain reaction (PCR). The relationship between human biting rate (HBR) and environmental variables was analysed using Spearman's rank correlation. A b A total of 214 Anopheles mosquitoes were collected, comprising 193 Anopheles introlatus (90.2%) 18 Anopheles maculatus (8.4%) and 3 Anopheles cracens (14%) Anopheles introlatus was the predominant species throughout the sampling period, with the highest abundance recorded in October (nm41), while the highest h at HBR w I was c observed in July (0.28 bites/ man/hour). The correlation analysis indicated that HBR increased with higher temperatures and wind speeds b s but decreased with increasing rainfall and relative humidity. This pattern suggests that climatic variables may contribute to variations in mosquito biting activity and seasonal abundance Futhermore, molecular analysis detected Plasmodium knowlesi and Plasmodiure coatney in An introlatus, confirming its involvement in parasite transmission. These findings indicate that An introlatus is the dominant species within the Leuccaphyrus group in Hutan Simpan Sempam and a potential vector of zoonotic malaria in Raub, Pahang, providing baseline evidence for future vector surveillance and targeted malaria control strategies

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

POSTER PRESENTATION ABSTRACT

P03

INTEGRATING GEMINI GEMS AI TO ENHANCE MOSQUITO IDENTIFICATION IN HIGHER EDUCATION: THE HEXAVECTOR UKM APPLICATION

Aishah Hani AZIL*, Muhammad Luqman NASARUDDIN

Department of Parasitology and Medical Entoreslogy Faculty of Medicine Universe

Chess Kale Langpur Malaysi

'Department of Bischisty Faculty of Medicine, Universis Kebangsaan Malaysia Cheras, Kuala Lump

ABSTRACT

Der Poster Presen

Identifying mosquito genera and species is a fundamental competency for undergraduate and postgraduate students in medical entomology. Conventionally, this skill is acquired through established taxonomic or pictorial keys, which can be time-consuming and discouraging for learners. To streamline laboratory sessions, instructors often provide supplementary handouts detailing key morphological features. With the rapid advancement of artificial intelligence (AI). educators are increasingly integrating this technology to enrich the teaching and learning experience. In this study we developed HexaVector UKM a Gems application powered by the Gemini Pro platform. This tool is designed to facilitate the identification of six medically important mosquito genera: Aedes, Anopheles, Cules, Mansonia, Armigeres, and Coquillettidia The application's knowledge base was synthesized from local taxonomic keys, with Al utilized to structure and guide the identification instructions. Preliminary internal testing provided initial evidence of HexaVector's utility as a supplementary tool in a medical entomology practical, demonstrating its feasibility for analysing physical specimens and diagnostic microphotographs. While promising, further refinement and testing are required to scale the application for broader implementation. In conclusion, such integration of traditional methods with advanced Al techniques offers extensive possibilities for enhancing medical and health- related education in higher learning institutions.

Keywords: mosquito identification, taxonomic keys Generative Al (Gems) GeminiPradalogy

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

DISTRIBUTION OF ANOPHELES VECTOR IN A PLASMODIUM KNOWLESI RISK AREA IN HUTAN SIMPAN KAHANG, KLUANG, JOHOR, MALAYSIA

Fadilawati ALI", Nor Hana AHMAD BAHURI, Ropiah JARA'EE

Wang Health District Office Kangoo Ma hor State Health Department, Johor Bahn Joer Malaysi "why

ABSTRACT

P04

Zoonotic malaria caused by Plasmodius knowlesi remains a public health concem in Malaysia, particularly in forested areas that serve as potential habitats for malaria vectors. This study was conducted in Hutan Simpan Kahang Kluang, Johor, following the report of two P. knowlesi cases, to determine the distribution of Anopheles maculatus and Anopheles leucophyrus as malaria vectors in the area. Adult mosquito collections were conducted from January to April 2020 using the human landing catch (HLC) method, while larval surveys were carried out at potential breeding sites. A total of 71 adult Anopheles mosquitoes were collected, consisting of An maculatus (n45; 63.4%), An. leucosphyrus (8: 11.3%), and Anopheles spp. (n=18: 25.3%). Anopheles maculatus was the predominant species, with the highest number recorded in April. The presence of An. leucosphyrus, a known vector associated with zoonotic malaria transmission, was also detected. Larvae of several Anopheles species including An. maculatus, An leucosphyrus. An sinensis, and An, kochi, were collected during the larval survey.indicating active breeding habitats within the study area. There was no consistent linear correlation between total rainfall and the general abundance of Anopheles mosquitoes. These findings highlight the potential risk of P. knowlesi transmission in Hutan Simpan Kahang Kluang, and support the need for continuous entomological surveillance and targeted vector control strategies.

Keywords: Plesire nelesi Anopheles maculetus Anopheles leucasphemus Malaysia haren landing catch (HLC), larval survey

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

POSTER PRESENTATION ABSTRACT

EMPOWERING COMMUNITIES TO BREAK THE DENGUE TRANSMISSION CHAIN IN SELANGOR, MALAYSIA (2018-2025)

• Khadijah KHAIRUDDIN, Faridah AMIN, Zaharah ZAINUDDIN
• ABSTRACT
• PO5

Deral Poster Presen

Despite the implementation of conventional vector control paradigms, recurrent urban dengue outbreaks in Selangor, Malaysia, necessitated sustainable community-driven interventions. Consequently the Community Sticky Ovitrap (CSO) programme was pioneered within the Petaling District from 2018 to 2025 to address this pressing public health challenge. The CSO is an award-winning, highly cost-effective lethal ovitrap (unit cost: RM 2.40) engineered to disrupt the vector life cycle. Utilising a dark container, dechlorinated water and a transparent adhesive sheet, the device captures and exterminates gravid female Aedes mosquitoes, thereby preventing oviposition and suppressing viral transmission. Deployed across persistent hotspot localities, the intervention was managed longitudinally by trained community volunteers who executed weekly installation and monitoring protocols. A Sticky Trap Index (STI) threshold exceeding 5% served as an entomologically early warning indicator, systematically triggering community-led mitigation frameworks. These included source reduction 'Search and Destroy' operations, targeted larviciding, and communal sanitation (gotong-royong) Longitudinal surveilance data demonstrated substantial epidemiological efficacy. Most notably, at the Rumah Pangsa Sri Saujana locality, the annual report of dengue incidence plummeted from 13 cases in 2018 to zero consecutively from 2020 through 2025. By transitioning a traditional entomological surveillance tool into an active, community-managed lethal control mechanism, the CSO programme establishes a sustainable, scalable, and evidence-based paradigm. This strategy effectively decentralises vector management, empowering local populations to break the dengue transmission chain.

Keywords: dengue prevention, Aedes, community empowerment, lethal op

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

P06

SMALL FLIES, BIG RISKS: TACKLING DROSOPHILA INFESTATION IN A HEALTHCARE FACILITY IN KOTA SETAR, KEDAH

Nurul Farhanah MOHD NASIR, Fatin Aina ABU BAKAR, Mohd Zukri IBRAHIM

Poster Presenc

Kata Sara Di Meath Of State Health Department, Alor Setar, K

ABSTRACT

Drosophila sp. a fruit fly species, can act as a transmission vector causing infestation and food contamination, particularly in healthcare settings. This study aimed to determine the source of Drosophila infestations within the food preparation and waste management areas of a healthcare facility in Kota Setar, Kedah Potential Drosophila breeding sites in these areas were inspected and recorded via physical observation. In 2024, positive breeding activity was observed exclusively in two site types, namely conveyor food trays and channel drains. Conveyor food trays recorded one positive site out of two inspected, representing a 50% infestation rate. Similarly channel drains accounted for one positive site out of five inspected, yielding a 20% rate. By 2025, the breeding distribution had completely shifted. Inspections of both conveyor food trays and channel drains recorded a 0% positivity rate, despite the number of inspected channel drains doubling to ten. All active breeding in 2025 was discovered in newly introduced inspection categories. Specifically, two positive sites were identified out of ten inspected drain covers, yielding a 20% rate, and one positive site was found of six inspected waste bins, reflecting a 17% rate. Targeted sanitation and environmental management succmsfully eliminated the primary breading grounds. However, the data reveal that pests adapt by migrating to secondary previously unmonitored habitats such as drain covers and the waste bin management area. To effectively suppress infestations, vector control strategies must be dynamic, employing continuously expanding surveillance to eliminate new reservoirs as they emerge.

Keywords: Drosophila, healthcare facility breeding sites, sector control strategies, food preparation area waste

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

POSTER PRESENTATION ABSTRACT

P07

ENHANCING THE EFFICACY OF RODENT CONTROL IN A HEALTHCARE FACILITY IN SOUTHWEST PENANG THROUGH MULTI-INTERVENTION STRATEGIES:

FINDINGS FROM OCTOBER 2024 TO MAY 2026

Wong Qi CHEN", Chiew Wye LEE, Ramalingam A/L THULASIRAMAN

Southwest District Health Department Balk Pul Penang Malaysia

ABSTRACT

Deral Poster Presen

Rodents easily adapt to human environments, causing structural damage, contaminating food, and spreading severe diseases such as hantavirus and leptospirosis. Their presence poses critical health risks, making sustainable control measures an absolute necessity. This study conducted at a healthcare facility which experienced rat infestations in October 2024 and January 2025, a shaon likely exacerbated by a nearby food court. This study intended to improve the effectiveness of rodent control interventions in a healthcare facility to achieve sustainable, long-term management Initially, signs of rodent activity such as droppings, grease marks, pathways, footprints, and structural cracks were recorded. Sicky traps and open rodent traps were placed indoors based on the observed signs of rodent activity. Although two rodents were caught, this single-method approach proved unsustainable, with infestations recuming two months post-treatment Consequently, a multi- intervention strategy was implemented. In addition to indoor trapping ultraviolet fluorescent tracking gel was applied to rodent pathways such as piping systems, and rodenticide was deployed within four Tamper Resistant Bait Stations (TBS) along the perimeter of the premises to establish a butter zone. The fluorescent gel revealed invisible rodent footprints and pathways, increasing detection by approximately 30% and providing crucial clues to optimise trap placement. The consumption of exterior rodericide confirmed outdoor rodent activity and successfully prevented rodents from infiltrating the building. This integrated approach substantially improved rodent control preventing re-infestation for up to 12 months since May 2005. These findings suggest that combining fluorescent tracking targeted trapping and perimeter baiting enhances the efficacy and sustainability of rodent management in healthcare settings.

Keywords: rodent control, healthcare facility, fluorescent tracking buffer zone, tablement

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

POB

THE FRONTLINE DEFENCE: MAPPING THE ADOPTION OF FOUR KEY COMMUNITY-LED MOSQUITO PREVENTION MEASURES IN MALAYSIA

Mohd Amierul Fikri MAHMUD, Amiru Hariz AMINUDDIN, Norzawati YOEP

Institute for Public Health Naissantes of Health, Minity of Health Malaysia Shalalang Malaysi Parks@mohgory

ABSTRACT

Dengue remains a significant public health challenge in Malaysia. While thermal fogging is common, sustainable management relies on active community participation. This study evaluates the adoption of four primary household-level vector control (HVC) interventions: larviciding, mosquito coib/electric masquito killers, serosal sprays, and repellents. These methods represent the four pillars of domestic protection: larval control, spatial repellence. adult knockdown, and personal protection. Data from the National Health and Morbidity Survey 2020 Communicable Diseases (Cognitive, Affective, and Behaviour] component were analysed (n=4,470). A nationally representative sample completed a validated self- administered questionnaire. Complex sample analysis was used to determine prevalence rates and 95% confidence intervals (CI) across sociodemographic characteristics. The national prevalence for HVC interventions was high: larviciding (82.3%, 95% CI: 872-913), mosquito cols/electric killers (86.8%, 95% CI: 84.5-88.9), aerosol sprays (85.7%, 95% CI: 82.7-88.2), and repellents (73.0%, 95% C 75.8-818). Respondents with a tertiary education demonstrated a significantly higher prevalence of aerosol spray usage (27.9%, 95% CI: 251-30.3] than those with no formal education (10.4%, 95% CI: 8.5-12.7). Furthermore, urban residents accounted for the highest larviciding efforts (675%, 95% CI: 62.8-719), compared with rural residents (21.8%, 95% CI: 17.8-26.4). The high prevalence of chemical-based interventions suggests a chemically proactive community; however, the reliance on education for aerosol and repellent adoption points to a knowledge-action gap. Future community engagement programmes should pivot from focusing on 'what to do, to 'how' to do it, ensuring that mosquito prevention measures become integrated as essential household habits

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Keywords most prevention, vector control community prevention

POSTER PRESENTATION ABSTRACT

P09

PROTECTING THE MILESTONE: RISK MAPPING FOR MALARIA RE-INTRODUCTION IN POST-ELIMINATION

MALAYSIA

Norzawati YOEP", Mohd Amierul Fikri MAHMUD, Mohd Amiru Hariz AMINUDDIN', Nur Ziana ABDULLAH SANI

Institute for Public Health National Institute of Health (NIH) Ministry of Health Malaysia Sea Alam Selang Mas Entomology & Pest Sets Disease Control Division, Minstry of Health Malaya

ABSTRACT

Deral Poster Presen

The global malaria situation remains a massive public health crisis. There are over 280 million cases and roughly 610,000 deaths annually worldwide. In 2018, Malaysia achieved a monumental public health milestone: zero local cases of human malaria. This success was hard-won through decades of relentless entomological surveillance, vector control and community cooperation Despite this achievement, a comprehensive spatial risk stratification framework integrating vector receptivity and population vulnerability remains unavailable. This study aims to geographically map areas based on their Malaria Re-introduction Risk (MRR). Data from 2,775 locations across Malaysia between 2019 and 2023 were analyzed using the Ministry of Health's MyEntoPest database. By assessing each area's receptivity, R (the presence and abundance of competent Anopheles vectors) and vulnerability. V (the influx of potentially infected populations), we calculated the specific MRR for each site (MRR=Rx V) Locations were then categorized and mapped as Low (MRR-12), Medium (MRR=3.4), or High (MRR-63) using GIS software. Over the five-year period, the vast majority of evaluated locations remained at Low risk for re-introduction. Furthermore, temporal mapping revealed a consistent, nationwide decline in High-risk areas. This reduction demonstrates that Malaysia's angoing vector control and surveillance strategies are effectively mitigating transmission risk. Ultimately, sustained entomological surveillance and vector control remain critical to prevent malaria re-introduction despite low risks

Keywords: malaria, malaria re-introduction risk, entomological surveillans, GS, M

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

P010

ASSESSMENT OF POST-OUTBREAK DENGUE VECTOR DENSITY USING THE STICKY OVITRAP INDEX (SOI) IN VILLAGE LOCALITIES OF MELAKA TENGAH DISTRICT, MELAKA

Sharifah Nur Shahirah SYED ABDUL HAMID, Ridzuan JANUDIN', Hana Fateha HASHIM', Zaitie Fatiha ZAINAL ABIDIN', Rabizah HAMZAH'

Mewa Tengah Distvist Office Melaka "Melaka State Health Department Melaka Melaka Malaysia hahahah@mohgowy

ABSTRACT

Vector surveillance using sticky ovitraps (50) is essential for evaluating dengue vector persistence during and after outbreak response operations. This study assessed the effectiveness of outbreak control activities by comparing the Sticky Ovitrap Index (SO) between active outbreak and post-outbreak phases in village localities of Melaka Tengah District. A longitudinal entomological surveillance study was conducted across 16 dengue outbreak village localities. SD deployment was performed systematically over four consecutive surveilance rounds. Rounds 1 and 2 represented the active outbreak phase, while Rounds 3 and 4 represented the post-outbreak phase. Mean SOI values were calculated and stratified into risk categories: low (<10), medium (010-0.30), and high (>0.30). A paired-samples t-test was applied to determine significant differences between the two operational phases. Statistical analysis demonstrated no significant reduction in vector density after the outbreaks were declared resolved (115)=-1282, p = 0.219). The overall mean S01 increased from 0.33 during the outbreak phase to 0.37 post-outbreak. High-risk localities expanded from 10 to 13 areas, representing 81.3% of the evaluated villages. Kampung Paya ikan recorded the highest increase (37.0%), reaching a peak SOI of 0.60. No locality successfully transitioned into the low-risk category. The persistently high Aedes deraity indicates that conventional adulticiding provides only temporary control without eliminating breeding sites. Strengthening integrated vector management (IVM) through multi-agency collaboration, targeted larviciding. environmental management, and community empowerment is crucial to prevent recurrent dengue transmission in village localities of Melaka Tengah.

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Keywords: sticky avtrap, dengue vector, Aedes, integrated vector management (VM)

POSTER PRESENTATION ABSTRACT

P11

DISRUPTING THE CYCLE: FIELD EFFICACY AND ENTOMOLOGICAL EVALUATION OF THE AEDES TECH MOSQUITO HOME SYSTEM (AMHS) IN JOHOR BAHRU, MALAYSIA

Siti Saodah IBRAHIM, Emira Izzati ABDUL AZIZ, Haidar Rizal TOHA', Ropish JARA'EE²

Vahor Bahru District Health Office Johar Baba Johor, Malaya "Johor State Health Department, lahor Bahru, Johor, Malaya "[email protected]

ABSTRACT

Deral Poster Presen

Dengue fever remains a major public health concern in Malaysia, with Johor consistently recording among the highest cumulative dengue cases nationwide. Johor Bahru contributes a substantial proportion of dengue cases within the state, highlighting the need for more sustainable and effective vector control strategies beyond conventional approaches. This study evaluated the field efficacy and entomological impact of the Andes Tech Mosquito Home System (AMHS) in reducing Aedes mosquito populations within dengue outbreak localities in Johor Bahru The AMHS is an eco-friendly autodissemination system developed as an alternative intervention within the integrated vector management (IVM) approach. Baseline entomological surveillance was conducted using conventional ovitraps during the pre- intervention phase, followed by ovitrap monitoring at the 18th week (mid-intervention). The AMHS was then deployed for 36 weeks, followed by post-intervention ovitrap surveillance Entomological parameters, including the Ovitrap Index (01), AMHS Index, Hatching Index (HI), and adult emergence percentage, were evaluated at six-week intervals. Data were analysed using paired sample t-tests with statistical significance set at p50.05. The results showed a significant reduction (ps0.05) in mosquito population density between the pre- and post-intervention phases. The Hatching Index in the AMHS was significantly lower [ps0.06) compared with conventional evitraps. In addition, the majority of mosquito eggs collected from the AMHS failed to develop into adult mosquitoes, indicating the effective inhibition of adult emergence. These findings indicate that the AMHS is effective in suppressing Aedes mosquito populations in dengue outbreak areas in Johor Bahru. The AMHS possesses strong potential as a sustainable and environmentally friendly supplementary vector control intervention to support dengue prevention and IVM programmes.

Keywords: Aedes Tech Mosquito Horse System (AMS), integrated vector management (VM), Aedes population, Ovitap Index vector contrel

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCP) 2005

POSTER PRESENTATION ABSTRACT

P012

LYMPHATIC FILARIASIS VECTORS IN SOUTHEAST ASIA: A SCOPING REVIEW OF RESEARCH GAPS AND METHODOLOGICAL TRENDS

Mohd Amiru Hariz AMINUDDIN", Faizul Akmal ABDUL RAHIM, Mohd Amierul Fikri MAHMUD, Khairul Aizat MAHDIN, Mohd Hazrin

HASHIM

Institute for Public Health National Insites of Heath Ministry of Health Malaysia Shah Abes Selangor Malaysi

ABSTRACT

This scoping review provides a comprehensive evaluation of lymphatic filariasis vectors within the Southeast Asian region from 2000 to 2025. The research identifies a significant geographical bias, as over 80% of the identified studies originated from Thailand and Indonesia, leaving major data gaps in other neighbouring countries. Methodologically, the literature reveals that the human landing catch method remains the primary approach for sampling mosquitoes. while dissection is the most common technique for detecting parasites. The authors highlight the need for integrated vector management and more modem surveillance technologies to meet the World Health Organization (WHO) 2030 elimination targets. Ultimately, these findings aim to guide regional surveilance by mapping the behaviours and habitats of five distinct mosquito genera responsible for disease transmission.

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Keywords: lymphatic latiasis, ectans, scoping reviews, Southeast Asia

POSTER PRESENTATION ABSTRACT

UNVEILING PERPETRATOR BEHAVIOUR: DIFFERENTIAL FLY COLONISATION ON DISMEMBERED HUMAN REMAINS IN MALAYSIA

P13

Syamsa Rizal ABDULLAH, Faridah MOHD NOR, Ahmad Firdaus MOHD SALLEH', Baharudin OMAR

Department of Parasitology and Medical Entomology Faculty of Medic Universi Kebangsace Malesia Cheras, Kala Lumpur Malaysia

Forensic Unit Department of Patology, Hospital Canselor Toanko Miz Cheras, Kala Lumpur M Mycology Unit Department of Diagnostic Laboratory Services Hospital Canselor Tanko Muniz Universi Kebangsaan Malaysia Kuala Langer Malaysi

Programme of Biomedical Sciences, School of Diagnostic & Applied Health Sciences

• Universiti Kebangsaan Malaysia Kuala L
• *undumy
• ABSTRACT

Deral Poster Presen

Forensic entomology plays a critical role in medicolegal investigations, particularly for estimating the minimum post-mortem interval (mPMI) in cases involving advanced decomposition and dismemberment. This case report presents a complex forensic case from Kuala Lumpur, Malaysia, demonstrating the utility of insect colonization patterna in reconstructing perpetrator behaviour. The case involves a 35-year-old female victim who suffered fatal blunt force trauma and post-mortem dismemberment. Her decapitated head was discovered concealed in a bolster, while other severed body parts were hidden within various wrappings in an apartment. Entomological examination revealed a significant disparity in fly colonization across the remains. The head hosted six diverse Dipteran species: Chrysomy megacephala, Chrysomya rufifacies, Chrysomya nigripes, Lucilia cuprina, Hydrotzea spinigera and Sarcophagidae. Conversely, the concealed body parts were colonized by only two species, Ch. megacephala and Ch. rufifacies. This differential colonization indicates the head remained exposed for a longer duration, permitting multiple oviposition events, whereas the perpetrator's activities on other body parts delayed insect access. Based on the developmental stages of Ch.megacephala collected from the head, the mPMI was estimated at approximately five days. corroborating subsequent police findings. The temporal separation in colonization provided a crucial timeline, suggesting sequential processing and concealment of the remains. This case underscores the importance of detailed entomological analysis for providing reliable biological timelines and behavioural inferences in challenging scenarios designed to obscure forensic evidence

Keywords: forensic entomology dismemberment case, post-morters interval, Malaysia, Chrysom megacephala

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

P010

THE POWER OF STICKY TRAPS: BREAKING THE DENGUE TRANSMISSION CHAIN VIA ADULT AEDES MORTALITY

Khuzaimah MOHD SAHIB', Nik Zarita NIK COB', Wan Mazlina WAN ABLAH', Khozah SAAD, Sarimah AHMAD', Fazliza KHAMIS, Khadijah KHAIRUDDIN**

Husah Pangse Sri Sagana Shab Alam, Selango

Entomology and Pest Unit Selangor State Health Deportent Sang Ma "@mich.goury

ABSTRACT

Conventional vector surveillance, such as using the standard ovitrap, primarily monitors larval breeding rather than actively reducing populations. Addressing persistent dengue outbreaks in Selangor, particularly in Rumah Pangaa Sri Saujana, the Community Sticky Ovitrap (CSO) was developed. This study evaluates the effectiveness of low-cost sticky traps in disrupting the dengue transmission by targeting adult Aedes mosquitoes. The CSO utilises a dark, water- filed container lined with a transparent sheet coated with Neopeace (Agricultural Chemicals (M) Sdn. Bhd.) a non-toxic aerosol adhesive. From 2018 to 2025, trained volunteers across high-density hotspots led the implementation. Each RM1.00 trap was inspected weekly. Aedes mortality data were reported via digital platforma, and a Sticky Trap Index (ST) exceeding 5% triggered immediate community-led "Search & Destroy" operations. The intervention proved highly cost-effective; a single RM 75.00 can coat 900 sheets, minimising restocking and ensuring an economical unit cost. Field data from Rumah Pangsa Sri Saujana demonstrated a significant correlation between trap deployment and disease reduction; reported dengue cases plummeted from 13 cases in 2018 to zero consecutively between 2020 and 2025. Weekly observations consistently recorded the neutralisation of hundreds of gravid Aedes aegypti and Aedes albopictus, successfully preventing oviposition and viral transmission. The sticky traps' efficacy lies in their dual action as a surveillance tool and a lethal control measure. By eliminating virus-carrying adult mosquitoes before they can bite or breed, the CSO immediately breaks the transmission cycle, offering a sustainable, community-driven and scalable model for urban dengue management in Malaysia.

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Keywords: dengue, Aade, sticky trap, community participation, Neopeace

POSTER PRESENTATION ABSTRACT

P15

UNMASKING CRYPTIC BREEDING: INTEGRATED ENTOMOLOGICAL INTERVENTIONS IN A PROLONGED DENGUE HOTSPOT AT PERUMAHAN PGA KLUANG

Maziah ZAKARIYA", Fadilawati ALI", Nor Hana AHMAD BAHURI", Ropiah JARA'EE'

Wat Tinggi Health Distict Office Kota Tinggi Malaysi Kang Health Distict Oce Kluang ho Malesia shor State Health Department Johor Bahru Jo

ABSTRACT

Deral Poster Presen

As Aedes mosquitoes increasingly exploit cryptic and unconventional aquatic habitats, traditional vector control methods face unprecedented challenges. This study details the entomological investigations and intervention strategies implemented during a prolonged 45-day dengue hotspot (13 cumulative cases) at Perumahan PGA, spanning from October to November 2025. A multi-pronged vector management approach was executed Surveillance included entomological investigations (E), sticky cvitrap deployment, virus detection in mosquitoes (VDIM) via NS1 antigen kits, and post-spraying bioassays. Active interventions involved intensive larviciding with Vectabac WG in subterranean manholes and the deployment of 300 Aedes-tech Mosquito Home Systems (AMHS) across the affected residential blocks. An El of 98 houses revealed significant cryptic breeding of Aedes aegypti and Aedes albopictus in overlooked indoor sites, specifically sink pipes and toilet bowls, as well as in closed concrete drains VDIM analysis successfully identified dengue-positive adult Ae. aegypti circulating within the hotspot. While chemical spraying achieved up to 100% mortality in adult mosquito bioassays, ovitrap surveilance indicated resilient breeding activity (up to 37% positivity in Block D) sustained by inaccessible vacant properties and unmaintained roof gutters. Unconventional indoor cryptic breeding sites heavily sustain localised urban dengue outbreaks. Eradicating such hotspots necessitates a strategic shift towards targeting hidden habitats using persistent biological larviciding, innovative auto-dissemination traps such as AMHS, and strict community management of indoor water traps.

Keywords: cryptic breeding Aedes-tech Mosesite Home Systems, virus detection in mesquit entomological investigations [] dengue hotspot

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

P016

ABUNDANCE, DIVERSITY, AND DISTRIBUTION OF ANOPHELES MOSQUITOES ALONG THE MALAYSIA- THAILAND BORDER, JELI DISTRICT, KELANTAN, MALAYSIA, 2018-2025

Mohd Azmi NUR NADIA", Nik Adib NIK AIDA!, Mohd Kamaruzaman AHMAD ZULFAHMI, Muhammad Jaafar NORJAIZA², Mohd Noordin NUR AKMARINA² Sulaiman SURAYA³

"Tanah Merah District Health Office, Tanab Marab, Kalantan, Malaysia Kelantan State Heath Department, Kata hala Pasir Puteh Dict Heath Oice, Pasir Puteh, Kelantan, Malayala "nurada [email protected]

ABSTRACT

Despite significant reductions in malaria transmission through regional elimination programmes malaria remains a major vector-bome disease in Southeast Asia. The border areas between Malaysia and Thailand are of epidemiological concem due to frequent human movement, forest- related activities, and the presence of effective Anopheles spp. mosquito vectors. Entomological surveillance was conducted in Jeli District, Kelantan, Malaysia, covering eight localities within 5 km of the Malaysia-Thailand border The goal was to identify the species composition, abundance and diversity of Anopheles spp. mosquitoes in this area. Sampling took place over four consecutive daya and nights using human landing catchers, animal-baked traps, eating collections and larval surveys. Collected mosquitoes were morphologically identified to the species level. A total of 5,861 Anopheles mosquitoes representing 22 species were collected. The most abundant species were Anopheles Rochi in 1814), Anopheles maculatus in 16381 Anopheles barbirostris (n=631) and Anopheles seats (558) Conversely, species such as Anopheles sinensis and Anopheles paralise, were rarely encountered Diversity analysis showed moderate mosquito diversity, with a Shannon-Wiener Index of (1931) and a Simpson's diversity index of (1-D) of 0.7896. Species richness was moderate but unevenly distributed, being dominated by a few key species. The dominance of Anopheles kochi and Anopheles maculatus aligns with earlier studies in Malaysia and Thailand, where these mosquitoes thrive in forest edges, rural landscapes, and border areas. These findings highlight the epidemiological importance of bander regions as habitats for malaria vectors and emphasise the need for continuous surveillance to support malaria prevention and elimination efforts along the Malaysia-Thailand bander

Keywords: Angeles spp, malaria vectors entomological surveillance, species diversity, Meleys-Thond border

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

• POSTER PRESENTATION ABSTRACT
• P17
• INTERACTIONS:

DECIPHERING CLIMATE-VECTOR TEMPERATURE AND RAINFALL EFFECTS ON AEDES OVITRAP POSITIVITY AND OVIPOSITION ACTIVITY IN BANDAR BARU PASIR PUTEH, KELANTAN, MALAYSIA

Suraya SULAIMAN, Nur Nabila JUSOH', Norjaiza JAAFAR, Zawiyah DOLLAH'

Pasir Puteh Health District Office Past Pu

Entomology & Pest Unit Kelantan State Health Department Kora Bhara Kelantan

ABSTRACT

Deral Poster Presen

Aedes mosquitoes are the primary vectors of dengue. Ovitraps are widely used for the early detection and monitoring of Aedes populations. Meteorological factors, such as temperature and rainfall, play a crucial role in influencing Aades population dynamics. This study investigates the relationship between rainfall and temperature with the ovitrap index and mosquito egg counts in Bandar Baru Pasir Puteh, Kelantan, Malaysia. A cross-sectional study was conducted aver two years (2023-2024) deploying 3350 ovitraps. Meteorological data were obtained from the Meteorological Department of Malaysia. The dependent variables were the ovitap index and mosquito egg counts. Descriptive and correlation analyses were conducted at a significance level of p < 0.06 Aedes albopictus was found to be more prevalent than Aedes aegypti, with 18.458 and 6,689 larvae recorded, respectively. A moderate positive correlation was observed between the ovitrap index and temperature (r = 0.31, p = 0.0046), indicating that higher temperatures are associated with increased Aedes populations. However, no significant correlation was found between the evitrap index and rainfall (r = 0.05, p = 0.08). Egg counts showed a weak negative correlation with temperature (r = -0.18, p = 152 x 10") and no significant relationship with rainfall (r=-0.09, p=2.29 x 10). Ultimately, temperature was shown to significantly influence Andes populations, demonstrating a weak positive relationship with the ovitrap index and a negative correlation with egg counts. Rainfall had a minimal impact on mosquito abundance and egg production, underscoring the importance of temperature monitoring for effective vector control

Keywords: Aedes mosquitoes, vitrap index, temperature renal dengue

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

P018

BUZZING TOWARDS DANGER: POULTRY FARMS AS DRIVERS OF PUBLIC HEALTH RISK

Noorazyan HARIS, Maziah ZAKARIYA, Mohd Ridzuan MOHD LUTPI, Khairul Anuar MOHD KAMDI, Hazlin ABU BAKAR, Cheah Yong XIANG'

Tetonalagy and Pest List Segara District Health Office Segurat Johor, Malay Entomology and Past Lin, Kata Tinggi Dat Heath Office, Kata Ting, Segamat Death Oce, Sagarat, Johor, Malayala

"Kata Tinga Dibit Health Of Kata Tinggi, Ja

"noorayanharis@mohoumy

ABSTRACT

Synanthropic fies, particularly Musca domestica, are critical mechanical vectors for enteric pathogens and key indicators of environmental sanitation. This study evaluated fly density dynamics across domestic, commercial, and agricultural zones in the Kota Tinggi and Segamat districts of Johor Malaysia, to identify public health risk hotspots and guide vector control Fly densities were monitored using standardised fly index traps deployed across domestic, commercial, and agricultural zones, capturing data from multiple poultry farms across the two districts alongside neighbouring commercial and residential areas. Population scores were interpreted against established intervention thresholds: 0-2 (none), 2-5 (discretionary), 6-20 (periodic control, and >20 Gimmediate emergency control). Spatial analysis revealed significant variation in fly abundance, with agricultural zones consistently contributing the highest densities. The monitored poultry farms across both districts repeatedlywiggered emergency intervention thresholds (>20). Furthermore, adjacent commercial premises and residential areas were abo heavily implicated, facing severe fly nuisances driven by localised vector spillover from these agricultural sources into human activity zone. Multiple poultry farms across the two districts act as primary drivers of fly proliferation and represent a significant environmental health threat to surrounding communities. Utilizing standardised density thresholds provides an objective framework for risk prioritisation. Mitigating these public health risks requires strengthening integrated pest management (PM), upgrading farm sanitation, and establishing routine entomological surveillance.

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

Keywords: les poultry vector, surveillance, health, integrated pest management (PM)

POSTER PRESENTATION ABSTRACT

P19

EFFICACY AND RESIDUAL ACTIVITY OF PYRIPROXYFEN AGAINST ANOPHELES CRACENS: IMPLICATIONS FOR ZOONOTIC MALARIA VECTOR CONTROL IN MALAYSIA

Intan Adibah SALIM, Nurul Nadiha MUHD TAMRIN, Rajiv RAVI, Olawale Quazim JUNAID*

Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur Malaysia "[email protected]

ABSTRACT

Deral Poster Presen

Malaria remains a significant global health concern, with zoonotic malaria caused by Plasmodium knowles/increasingly reported in Malaysia. Conventional vector control strategies. such as long-lasting insecticide-treated nets (LLINS) and indoor residual spraying (IRS), have limited effectiveness against outdoor-biting Anopheles mosquitoes, necessitating alternative control approaches. This study evaluated the larvicidal efficacy of pyriproxyfen (PPF), an insect growth regulator, against laboratory-reared third-instar larvae of Anopheles craces, primary vector of P. knowlesi in Peninsular Malaysia. Laboratory bioassays were conducted to determine the concentrations required to achieve 50% and 90% adult emergence inhibition E and IE evaluate morphological alterations during larval and pupal development, and establish a diagnostic dosage. The study found that PPF effectively inhibited adult emergence, with IE and IE values of 0.0158 mg/L (95% CI 0.003-0.044 mg/L) and 3.35 mg/L [95% CI: 125-147 mg/L), respectively Distinct morphological abnormalities, including generalised dark pigmentation of larvae and pupae, red colouration in the early cephalic region, and abnormal cuticle deposition, were observed in An cracens. Based on probit analysis, the diagnostic dosage was determined and subsequently applied under simulated field conditions to assess the residual activity of PPF over time. Maximum residual larvicidal activity was sustained for up to four weeks under simulated field conditions. These findings indicate that PPF has strong potential as an effective larvicidal agent against An cracens and support its consideration in targeted vector control strategies to mitigate the rising burden of zoonotic malaria in Malaysia.

Keywords Anceles cracons, pyriprogfes, bioassey, vector centrol simulated field testing

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

P20

EVALUATING THE RESIDUAL EFFICACY AND STRAIN- DEPENDENT PERSISTENCE OF BIFENTHRIN SURFACE TREATMENTS AGAINST AEDES AEGYPTI

Fatin Nur Adlina ROZAIDI, Norlaili MISRAWI, Aimi Afiqah MOHAMMADIN, Ezita Qaisara RIDZUAN, Mohammad Farizul MOHAMMAD FATIHI, Haziq Uzair MOHD ROSLI, Afiq Asyraf MOHD FAIZOL, Farah Diana ARIFFIN, Nurulhusna AB HAMID

Medical Entomology Unit Infectious Disease Research Centre, Institute for Medical Research Nati of Health Ministry of Heal Selangor Malaysia

ABSTRACT

The operational effectiveness of residual insecticide applications against Aedes aegypti, the primary vector of dengue, is strongly determined by the concentration of the active ingredient retained within treated surfaces. This study aims to evaluate the effectiveness of a water-based suspension concentrate formulation of 10% w/v bilanthrin at two operational concentrations, C, (0.09%/active ingredient 1:200 dilution ratio) and C, 1010% w/v active ingredient: 1100 dilution ratio, comparing a laboratory strain (LS) and a field-collected wild strain (WS) under semi-held conditions using standard WHO cone bioassays. Adult female masquitoes were exposed to treated surfaces across three vertical resting heights (40 cm, 80 cm, and 120 cm), with 24-hour post-exposure mortality monitored over a 56-day evaluation cycle. Preliminary results demonstrate that efficacy depends heavily on concentration and the specific mosquito strain. At C, the laboratory strain exhibited high initial efficacy (85.00 ± 11.23% at Day 7; peak 6.67 ± 5.77% at Day 28] before dropping sharply to 10.97 80-40% by Day 56. Escalating the concentration to C, significantly extended residual longevity (7708± 15.71% at Day 56). In contrast, the wild strain at C, exhibited a low initial response (4.44 770% at Day 7) before rising to 63.77 ± 14.51% at Day 56. Doubling the concentration to C, succmfully overcame this slow start in the wild strain raising Day 7 mortality to 61.25 13.02%, despite cyclical dips at Day 14 (23.08 ± 1.36%) and Day 42 (25.77 ± 1.63%). Efficacy trends were generally comparable across all evaluated vertical resting heights. These findings demonstrate that evaluating only laboratory strains tends to overestimate initial field performance against wild populations. Optimising bentin application concentrations is critical to overcoming early response lags and securing sustainable vector suppression within broader dengue control programmes

Keywords: benthin, Aedes aegypt, cone bioassay, residual efficacy insecticide concentration, dengue vector management

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

POSTER PRESENTATION ABSTRACT

P21

EVALUATION OF DIFFERENT RODENTICIDE BAITS FOR RODENT MANAGEMENT IN CBU AND PIDM VEHICLE STORAGE AREAS AT TANJUNG MALIM, PERAK

Syazana ARIE", Ainas MARDIA

Department of Exto and Technical Tax Pest Control Sdn Bhd Batu Caves, Selangor, Mala "[email protected]

ABSTRACT

Deral Poster Presen

Rodents are small mammals belonging to the family Muridae and the order Rodentia, a group characterised by a pair of continuously growing incisors used for gnawing various materials, including electrical wiring systems in vehicles. Rodents are among the most significant vertebrate pests worldwide due to their remarkable adaptability to diverse environments and high reproductive potential. The primary objective of this study was to evaluate the effectiveness of different rodenticides based on the reduction of rodent activity. indicated by the presence of droppings, gnaw marks, and bait consumption. In addition, the study aimed to identify the most suitable rodenticide for use as part of an integrated Pest Management (PM) programme within a vehicle storage warehouse environment. The relationship between bait consumption and the reduction of both rodent populations and activity levels within the study area was also examined. Two rodenticides were evaluated: flocoumafen (0.005%) and bromadiolone (0.005%). The findings indicated that flocoumalen (0.006 %) was highly effective for controlling rodents in areas with high infestation levels compared to bromadiolone (0.005%). Conversely, bromadiolone (0.005%) was found to be more suitable for maintenance control programmes and locations with low rodent infestation levels. Overall, rodent control programmes utilising bromadiolone (0.005%) were determined to be more appropriate for long-term implementation within an Integrated Pest Management (IPM) framework, as this approach poses less disruption to the food chain and offers a more cost-effective solution compared to flacoumafen (0.005%).

Keywords: focoumen (0.006) broader (3009 rodent, rodenticide

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

P22

GENETIC DIVERSITY OF AEDES ALBOPICTUS IN KUALA TERENGGANU USING COX1 GENE SEQUENCE FOR SUSTAINABLE VECTOR CONTROL

Intan Balqis MOHD SATAR, Nur Aida HASHIM², Suzana MISBAH"

Faculty of Science and M

Universi Malaysia Terengganu, Kuala Nerus Teen Malaysia

Faculty of Food Science and Agrotechnology Universi Malaysia Terengganu Kaala Nerus Te

ABSTRACT

The increasing incidence of dengue in Malaysia highlights the need for effective and sustainable vector control strategies. Aedes albopictus is a major dengue vector widely distributed across urban and semi-urban areas; however, dengue incidence varies between localities despite its presence, possibly due to differences in the genetic composition of local mosquito populations. This study investigated the species identity and mitochondrial DNA sequence variation of Ae albopictus in Kuala Terengganu using an integrated morphological and molecular approach Adult mosquitoes were collected from a dengue hotspot area, Kg. Losong, and a low-incidence area, Universiti Malaysia Terengganu Species identification was initially performed based an morphological characteristics and subsequently confirmed by polymerase chain reaction (PCR) amplification of the mitochondrial cytochrome c oxidase subunit I (COX) gene. Initial PCR amplification produced double bonds, indicating non-specific amplification; therefore, the target fragment was excised and purified, yielding a single band of approximately 60 bp suitable for sequencing Sequence comparison and phylogenetic analysis showed that the Kuala Terengganu Ae. albopictus sequences clustered with Southeast Asian reference sequences, with no clear genetic differentiation observed between the two sampling sites. These findings indicate a genetically homogeneous Ae. albopictus population within the study area and provide baseline genetic information to support molecular surveilance and sustainable dengue vector control strategies in Malaysia.

Keywords: Aedes albopictus, genetic diversity mitochondrial COKI gene, phylogenetic analyst control

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

POSTER PRESENTATION ABSTRACT

P23

WHO'S BUZZING IN THE WETLAND? A THREE-YEAR LONGITUDINAL ANALYSIS OF MOSQUITO FAUNA (2023-2025)

Hanipah SHAHAR", Rafidah ALI", Manimegalai V', Rozita AR, Haliza AM', Helmi Ab HAMID

Enology and Pest Branch Kuala Lumpur & Pub

Federal Tanitary State Health Office Kivala Lon

Tropical ect Disease Research & Education Centre (TDRECL High pact Research Building (HRL Universi Melaye Kaala Lumpur Mal

Kale Campur & Phajaya Federal Temary State Health Offon Kala Lampu Malaysi Ensenal Health Division City Service Department Perbasane Puhaja Punya Malaysi

[email protected]

ABSTRACT

Uer of Poster Presenc

Sustained entomological surveillance is essential for understanding mosquito profiles to support evidence-based vector control strategies. This study aimed to characterise the mosquito species composition, relative abundance, and potential public health risks at Werland Park, Putrajaya. Adult mosquitoes were collected monthly (2023 to 2025) using human landing catch from 18:00 to 00:00 hours. Larval surveys were performed intermittently subject to site restoration activides Mosquitoes were morphologically identified, and data were analysed using appropriate statistical tools. A total of 8.031 mosquitoes, representing 14 species across four genera, were collected. The dominant species was Anopheles hyrcanus, followed by Anopheles barbirostris and Mansonia uniformis Three medically important species were identified: Aedes albopictus, Marsonia uniformis, and Culex quinquefasciatus. Humanlanding rate (HLR) were highest for Mansonia uniformis (3.4%), followed by Culex quinquefasciatus (0.52%) and Aedes albopictus (0.39%). A significant difference in abundance was observed among vector species (H-12.09, df=2, p=0.0021 with Mansonia uniformis showing the greatest abundance. No significant correlation was found between HLR and time, suggesting the absence of a distinct temporal biting pattern. Larval survey data were excluded due to a small sample size. These findings highlight the diversity of mosquito species at Welland Park. Although the estimated transmission risk was low to moderate, the presence of vector species indicates a potential risk for disease transmission Restoration activities may disrupt breeding sites, reducing larval survival and subsequent adult emergence. Future research should incorporate meteorological data, molecular species identification, and consistent larval sampling schedule to improve profiling and mosquito risk assessments.

Keywords: Culicidae, mosquito, abundance, public health importance

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

POLLINATION-BASED FLOWER MORPHOLOGY MATCHING WITH STINGLESS BEE AND VANILLIN CONTENT ANALYSIS IN CULTIVATED VANILLA SPECIES

Qistina JAMALUDIN*, Nurul Aliaa IDRIS

Faculty of Science & Maxine Environment Live Malay T Kuala Nerus Teangalaya *570034@cuted my

ABSTRACT

P24

Vanilla planifolia is one of the world's most valuable spice crops, cultivated globally due to its distinctive flavour and economic importance. Despite its widespread cultivation, vanilla production faces a major challenge regarding pollination, as effective natural pollinators remain uncertain outside its native range. Consequently vanilla cultivation relies heavily on manual pollination, which increases labour demands and production costs. This study first aimed to evaluate the potential of Malaysian stingless bees (Heterotrigona itama and Genietigona thoracica] as pollinators of K planifolia using a flower-pollinator morphology matching approach. Hierarchical clustering analysis revealed that functional bee traits, particularly body length and thorax width, were more closely associated with floral reproductive structures than head width. This suggests that pollination potential is driven by functional morphology rather than species identity. In addition to pollination constraints, vanilla quality and market grading are strongly influenced by vanilin content; however, global grading systems largely depend an physical pod characteristics that may not accurately reflect chemical quality. Therefore, this study also quantified and compared vanilin concentrations between V. planifolia and V tahiterais using high-performance liquid chromatography (HPLC). The results demonstrated that V. planifolia possessed significantly higher vanillin concentrations (24847 mg/L) than Vtatensis [125.81 mg/L), reflecting species-specific biochemical regulation and precursor accumulation. Overall, this study highlights the importance of integrating morphological assessments for pollinator identification with chemical analysis to improve vanilla quality evaluation and grading

Keywords: high-performance liquid chromatography HPLC) analysis, mohology matching stingless bees Vonilla plenifee Villas

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

POSTER PRESENTATION ABSTRACT

P25

COMPARATIVE DEVELOPMENTAL STABILITY OF CHRYSOMYA NIGRIPES AND BOETTCHERISCA HIGHLANDICA IN LONG-TERM LABORATORY COLONIES: IMPLICATIONS FOR FORENSIC CHRONOLOGY, PUBLIC HEALTH, AND URBAN SUSTAINABILITY

Hillarian MOJIUN, Roziah ALI", Bekcham Tan Chia HUNG, Khairul Asuad MOHAMED, Azahari ABD HADI, Johnnetha John GINDABAL, Novita AMHAR

Medical Entomology Unit, Infectious Disease Research Centre, Institute for Medical Resea of Health Ministry of Health, Selangor, Malaysia

ABSTRACT

Poster Presection

Accurate estimation of the minimum post-mortem interval (PMI) and monitoring disease vectors in Southeast Asia require precise developmental models for niche-specific flies. While common species like Chrysanya megacephala are well-documented, the late-colonising Chrysomya nigripes and the highland-specialist Boettcherisca highlandica remain under characterised, particularly regarding developmental stability across hundreds of generations. This study aimed to determine the developmental kinetics of C. nigripes and B.highlandica in standardised laboratory environments and evaluate the biological reliability of long-term colonies F31 and F12, respectively) for forensic and public health applications Laboratory colonies were maintained under controlled conditora (258TC and 70% 10 RH). Developmental durations for each life stage-egg-to-L. L1-to-L2 L2-to-L3, L3-10- pupa, and pupe-to-adult-were recorded across multiple filial generations. Data were synthesised to establish species-specific growth constants. Boettcherisca highlandica demonstrated a significantly more protracted lifecycle (maan 1755 1.5 days) compared to C. nigripes (mean 13.44 1.2 days). The most significant temporal divergence was observed during the intra-puparial period, where B. highlandica (7.85 days) exceeded C. nigripes (5.44 days) by over 44% Despite the high number of generations (F121 for B. highlandica), developmental rates remained remarkably consistent, supporting the validity of using established laboratory strains for forensic modelling. These findings provide essential baseline data for precise forensic reconstruction in tropical urban and montane ecosystems. Furthermore, the role of these species as bioindicators of environmental health and potential vectors for zoonotic pathogens highlights their significance in the broader context of ben sustainability and public health protection.

Keywords: Chrysonry nigripes Boenchesca highandica minimempost-mertens interval urban

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

P26

ENTOMOLOGICAL RISK ASSESSMENT (ERA) FOR DENGUE INFECTION IN CHERAS DISTRICT PUBLIC PLACES (2024-2025)

Nor Aszlina ISMAIL", Hanipah SHAHAR

Entomology & Pest Branch, Kuala Lumpur & Putrajaya Federal Territory State Health Office, Kuala Lumpur, Malaysia *[email protected]

Poster Presection

ABSTRACT

Dengue fever poses a significant public health threat in Malaysia, with the Federal Territory of Kuala Lumpur and Putrajaya (WPKL&P) contributing substantially to national cases. The Cheras District consistently reports a high percentage of dengue infections, accounting for 18.9% of total WPKL&P cases in 2004 and 15.9% as of epidemiological week 24 (EW24) in 2025. This study aimed to evaluate the risk of dengue virus transmission across 25 public places selected in Cheras District through an Entomological Risk Assessment (ERA) spanning 2024 to 2025. Aedes breeding inspections covered both indoor and outdoor environments at these locations. Collected specimens underwent laboratory species identification and larval staging The analysed data revealed that Andes breeding positivity in Cheras District public places increased from 24% in 2024 to 44% in 2025. The Bandar Tun Razak Parliament consistently exhibited higher positivity rates than the Cheras Parliament. Aedes albopictus was the predominant species, indicating outdoor breeding behaviours. Plastic containers were found to be the key containers (18.9%), indicating poor waste management practices. This ERA highlights public places in Cheras District, especially within Bandar Tun Razak Parliament, present a high risk for dengue infection. The escalating Aedes breeding in 2025, necessitates targeted monitoring. Aedes albopictus prevalence suggests abundant unmanaged man- made containers and poor waste management. The presence of late-stage larvae indicates persistent breeding sites. Increased surveilance and targeted preventive strategies are necessary to reduce the dengue transmission risks posed by public places in Cheras. Inter- agency collaboration, regular Aedes 'search and destroy' activities, stringent waste collection monitoring, and intensive health education for facility owners are crucial to mitigating dengue transmission risks.

Keywords public places Cheres District is, dengue transmission Entomological Risk Assessment (ERA)

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

POSTER PRESENTATION ABSTRACT

P27

INTRINSIC TOXICITY OF DELTAMETHRIN AGAINST AN ADULT AEDES AEGYPTI LABORATORY STRAIN VIA TOPICAL BIOASSAY

Nur Amalina KAMARUDIN, Farah Diana ARIFFIN, Azahari ABDUL HADI, Shazreena ROSLI, Khairul Asuad MUHAMED, Beckham Tan Chia HUNG, Nurul Fatihah MOHAMAD JAAFAR, Lukman Hakim HAIRUDDIN, Nur Hazwani BAKARUDDIN, Nur Farihah AB HAMID, Rosilawati RASLI

Medical Entology Unfectious Disease Research Centre (DRC) shute for Medical Research (WR) National ones of Health pH Ministry of Health Malaysia (MOH) Setia Alas Selangor Malaysia

ABSTRACT

Determining the intrinsic response of an Aedes aegypti laboratory strain to deltamethrin via topical bioassays isolates insecticide toxicity from confounding behavioural factors. This approach quantifies toxicity by ensuring direct exposure and full absorption of insecticides. This study evaluated the intrinsic response of adult female Ae. aegypt to a range of deltamethrin concentrations. In addition, the fold concentration of deltamethrin was determined by comparing the diagnostic dosage with the World Health Organization (WHO) discriminating concentration of deltamethrin. Baseline concentrations were prepared from technical-grade deltamethrin (90% purity), ranging from 0.0005 mg/L to 2 mg/L across ten doses. Tested doses were directly applied to the thorax of anaesthetised adult female Ae. aegypt (laboratory strain, aged 3 to 5 days) using a calibrated micropipette. Mortality was recorded after 24 hours. and probit analysis was performed to determine the LD, and LD, values. A dose-dependent mortality increase was observed, with concentrations ranging from 0.0006 mg/L to 0.0008 mg/L produced low mortality 0.005 mg/L to 0.008 mg/L produced moderate mortality, and 0.05 mg/L to 0.08 mg/L produced high mortality indicating a suitable range for probit analysis (chi-square, X 16.04, p < 0.005). The analysis yielded an LD value of 0.08 mg/L (95% CI: 0.027 mg/L -0.508 mg/L) and an LD, value of 4.97 mg/L (95% CI:0.67 mg/L - 103374.46 mg/L). This study provides a baseline reference for establishing concentration series in topical bioassays assessing the intrinsic toxicity of deltamethrin against Ae. aegypti. The resulting LD, serves as a comparative benchmark for other insecticides, whilst the LD,, can inform discriminating dosage determinations.

Keywords: intrinsic topical bioassay probit analysis

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2005

POSTER PRESENTATION ABSTRACT

P28

VECTOR COMPETENCE OF AEDES ALBOPICTUS IN VIRUS TRANSMISSION: A SYSTEMATIC LITERATURE

REVIEW

Ali ROZIAH***, Rasli ROSILAWATI, WAN Kiew-Lian'

Department of Biological Sciences and Boteco Faculty of Science and Technology, Univers "Medical Entomology Unit Infectious Disease Research Cante estitute for Medical Research of Health Ministry of Heal Selangor, Malaysia

Poster Presection Ab

ABSTRACT

Aedes albopictus (Asian tiger mosquito) has emerged as a significant vector of multiple arboviruses globally. Understanding its vector competence across different geographic regions and virus types is critical for predicting and managing arboviral disease outbreaks. This systematic review synthesises evidence on the vector competence of Andes albopictus for transmitting arboviruses. We conducted a comprehensive systematic Iterature review following PRISMA 2020 guidelines. Three databases were searched (PubMed, Scopus and Web of Science) covering publications from 2005 to 2025. Eligible studies assessed Aedes albopictus vector competence for viral pathogens using quantitative measures, including infection, dissemination, or transmission rates, and reported population-level variation Studies on non-viral pathogens or ecology alone were excluded. Of 1,240 records identified, 042 remained after duplicate removal. After screening 500 abstracts and assessing 157 full texts 145 studiare included. Most studies used laboratory experimental designs (n=138), with fewer field-based investigations CHIKK DENV, and ZIKV were the most frequently studied viruses, with mosquito populations from Europe, Asia, the Americas, and Africa. Vector competence varied by virus, mosquito population origin, and environmental conditions, particularly temperature. Aedes albopictus demonstrates high vector competence for CHIKV across multiple geographic regions, moderate to high competence for DENV depending on serotype and population, and variable competence for ZIKV Temperature significantly influences transmission dynamics, with higher temperatures generally enhancing vector competence Geographic variation in competence highlights the importance of local vector surveillance. A meta-analysis with a random-effect model was also conducted to assess publication bias. These findings have oftical implications for arboviral disease risk assessment and public health preparedness, particularly in temperate regions experiencing Aedes albopics invasion

Keywords: Aedes albopictus, invasive species, vector-bene dieses Zia virus (ZKV), dengue virus (DENV) Chikungunya virus (DHKV]

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

POSTER PRESENTATION ABSTRACT

COMPARISON OF THE RESIDUAL EFFICACY OF LAMBDA CYHALOTHRIN CAPSULE SUSPENSION AGAINST Aedes AEGYPTI BETWEEN TWO SPRAY CYCLES

P29

Aimi Afiqah MOHAMMADIN, Norlaili MISRAWI, Fatin Nur Adlina ROZAIDI, Ezita Qaisara RIDZUAN, Mohammad Farizul MOHAMMAD FATIHI, Haziq Uzair MOHD ROSLI, Afiq Asyraf MOHD FAIZOL, Farah Diana ARIFFIN, Nurulhusna AB HAMID*

Medical Entomology Unit Infectious Diseasearch Canha, Institute for Medical Research, Nationa of Health, Ministry of Health, Selangor, Malaysia usne [email protected]

ABSTRACT

Poster Presection Ab

The residual performance of insecticides is a key determinant of their effectiveness in vector control programmes, particularly for managing Aedes aegypti, the primary vector of dengue transmission. This study evaluated the residual bio-efficacy of a capsule suspension formulation against laboratory strain (LS) and wild strain (WS) Aedes aegypti under semi-field conditions using the standard WHO cone bioassay Adult mosquitoes were exposed to treated surfaces at three domestic resting heights (40 cm 80 cm, and 120 cm), and knockdown and 24-hour mortality responses were monitored across two application cycles over an extended period. The results demonstrated marked strain-dependent variation in residual efficacy. For the laboratory strain, the capsule suspension maintained high residual efficacy across bath cycles, reaching a peak mortality of 37.55 ± 2.05% by Day 42 in Cycle 1 and sustaining 100% average mortality through Day 70 in Cycle 2. with residual performance remaining above 68% up to Day 140 (68.52 ± 5.86%). In contrast, the wild strain exhibited substantially lower and less predictable susceptibility in Cycle 1, wild strain mortality remained low (1.23% ±214% on Day 28 to 11.60 ± 2.37% on Day 70] across most intervals despite a transient peak at Day 42 (98.89 ± 1.92%), while Cycle 2 showed a gradual increase in mortality peaking at Day 56 (28.9 ± 175%) before declining to 3410 ± 8.08% by Day 140. Efficacy trends were generally comparable across the evaluated resting heights without significant height-dependent variations. These findings indicate that while the capsule suspension provides extended residual control against susceptible laboratory populations, wild populations display high variability and reduced susceptibility under semi-field conditions, highlighting the essential need to evaluate vector control formulations against local wild strains when assessing residual spray efficacy

Keywords: capul suspension, Aedes aegypt, cone bioassay maidual longevity, insecticide resistance, vector control

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCP) 2005

POSTER PRESENTATION ABSTRACT

P30

LABORATORY OBSERVATIONS ON OOTHECAL FORMATION AND NYMPHAL EMERGENCE IN GERMAN COCKROACHES (BLATTELLA GERMANICA)

Ezita Qaisara RIDZUAN', Nur Amalina KAMARUDIN', Noor Darliah SIKANDAR, Norlaili MISRAWI', Mohammad Farizul MOHAMMAD FATIHI, Fatin Nur Adlina ROZAIDI', Aimi Afiqah MOHAMMADIN', Nurulhusna AB HAMID

'Medical Extralay Unit, Infectious Disease Research Centre, tute for Medical Research, National of Health, Selangor, Malayala

Entomology and Pest Section, Tawau District Health Office Chang Thain Road, Taa Sabab na [email protected]

Peter Presections

ABSTRACT

Infestations of the German cockroach (Blattella germanical present significant public health concerns due to its role as vectors of pathogens linked to food contamination. The need to understand their life cycle and reproductive biology is crucial to support effective biological research and pest control strategies. This study aimed to optimise colony management through evaluation of reproductive performance, offspring production and survivability of B. germanica under controlled laboratory conditions. Individual females were paired with a single male and monitored from adult emergence until death Parameters observed included adult female span, ootheca production and incubation period, nymphal development duration, and offspring viability Results showed that adult females lived for an average of 9118 28.84 days and produced 344100 oothecae during their lifetime, with an average of 35.34 ± 9.96 successfully emerged nympia per female. Overall hatchability demonstrated strong reproductive viability (103 viable vs. 28 non-viable oothecael Nymphal development to full adulthood required an average of 42.75 ± 845 days, resulting in 3184 ±6.52 surviving adult afspring per female with an almost equal sex distribution (18.21 6.48 males to 1663-365 females). The high egg viability rate [+78.6%) and rapid nymphal development time (42.75 8.46 days) underscores the reproductive efficiency of this species. These baseline quantitative data contribute to population dynamics modeling and provide practical, mechanism-based guidelines that enhance the efficiency reproducibility, and biological integrity of B. germanica colony management for research and pest management applications

Keywords: Bello germanica File table reproduction, fecundity survivability colony mangement

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCEP) 2006

POSTER PRESENTATION ABSTRACT

P31

COMPARATIVE RESIDUAL EFFICACY OF THREE DELTAMETHRIN FORMULATIONS AGAINST LABORATORY AND WILD STRAINS OF AEDES AEGYPTI UNDER SEMI-FIELD CONDITIONS

Norlaili MISRAWL Aimi Afiqah MOHAMMADIN, Fatin Nur Adlina ROZAIDI, Ezita Qaisara RIDZUAN, Mohammad Farizul MOHAMMAD FATIHI, Haziq Uzair MOHD ROSLI, Afiq Asyraf MOHD FAIZOL, Farah Diana ARIFFIN, Nurulhusna AB HAMID*

Medical Entomology Unit Infectious Dise Research Centre, Institute for Medical Research of Health, Ministry of Health, Selangor, Malaya

ABSTRACT

This study evaluated the residual efficacy of three different deltamethrin formulations comprising a liquid suspension, wettable granules, and a microencapsulated formulason against laboratory strains (LS) and field-collected wild strains (WS) of Aedes aegypti under semi-held conditions using standard WHO cone bioassays across three vertical indoor resting heights up to Day 112. When evaluated against the laboratory strain (LS), all applicable formulations demonstrated consistently high susceptibility and robust control through Day 112, the liquid suspension and wettable granules maintained exceptionally high, stable control through Day 84 with mortalities reaching 100% before declining by Day 112 (72.22± 14.98% for liquid suspersion and 889 ± 1.92% for wettable gram) while the microencapsulated formulation sustained high control reaching 94.48 ± 3.47% by Day T12. In stark contrast, testing against the wild strain (WS) exposed severe performance variations that scientifically justify the necessity of Seld-strain evaluations. Specifically testing for the liquid suspension formulation was restricted exclusively to laboratory trials due to anticipated rapid eld degradation. Meanwhile, the wettable granules achieved high mortality spikes up to 100% on certain evaluation days (such as Day 42 and Day 112) and remained relatively stable without dropping below 50% mortality, though they still cohibited noticeable performance fluctuations across the evaluation period. Finally, the microencapsulated formulation utilized a specialized polymer layer covering the active ingredient, which inherently resulted in a delayed initial mortality response against the wild strain, showing lower figures on Day 7 (55.79 ± 8.34%) and Day 28 (5111 ± 12.62%), but its performance steadily climbed starting from Day 42 (76.22 ± 18.69%) and achieved robust vector suppression exceeding 50% mortality by Day 12 (reaching 78 ± 3.85%). Overall, residual efficacy differed among deltamethrin formulations and between laboratory and wild strains of Aedes aegyp demonstrating the importance of field-based evaluations for effective vector control

Keywords: Aedes aegypt, delta formulations, microencapsulation technology, semi-field evaluation WHO cone bioessays

INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST SCP) 2005

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