ISCEP 2026 At Sunway Putra Hotel 17-18 August 2026
ABSTRACT
ORAL PRESENTATION ABSTRACT
T1_05
EVALUATION OF TWO PROBE-BASED qPCR MASTER MIXES PERFORMANCE FOR FILARIAL DNA DETECTION IN MOSQUITO VECTORS
Masse RS", Tupang L², Jaafar AJ, Abdul Rahman NA', Jeyaprakasam NK, Mohd Ali MR
Wedical Entonalagy Unit fectious Disease Research Centre Insite for Medical Research (National Institutes of Heath (NH) Ministry of HeaMalesia
Entomology & Pest Brunch Sabah Health Department Kota Kinabalu Sabb
Center for Toxicology & Health Risk Studies (CORE) Faculty of Health Sciences Univers "Bactesy Unifectious Disease Research Centre, sue for Medical Research (WR) National of Heath H Mistry of Health Malaysia
Lymphatic filariasis (LF) is a mosquito-bome neglected tropical disease caused by Wuchereria bancroft, Brugia malay, and Brugia Timor and remains a significant public health burden in andemic tropical and subtropical regions. Molecular xanomonitoring using real-time PCR is a seraitive approach for detecting filarial DNA in mosquito vectors. This study evaluated the performance of two probe-based oPCR master mixes, TaqMan Fast Advanced Master Mix and SemiFAST™ Probe No-ROX Kit, for filarial detection in mosquito vectors. An initial comparison was conducted using four selected mosquito DNA samples collected from a filariasis-endemic area in Beaufort, Sabah. Performance was assessed based on threshold cycle (Ct) values and cost per reaction. Under the tested conditions, the SensiFAST Probe No ROK Kit produced lower Ct values than the TaqMan Fast Advanced Master Mix and showed better cost effectiveness. Based on these results, the SeraiFAST Probe No ROX Kit was used to screen 71 pooled field- collected mosquito samples from the same locality for filarial DNA detection targeting the 285 LSU rRNA gene. A total of 17 samples were positive for filarial DNA and were further analysed using a species-specific qPCR assay targeting the Hhal gene of Brugia malayi/Brugia timari Of these, four samples were confirmed positive, with infections detected in Mansonia uniformis (m3) and Mansonia bonneas (nm1). These findings suggest that the SansFAST Probe No- ROX Kit is a practical and cost-effective option for molecular xenomonitoring of LF vectors in andemic settings. Further validation with larger sample sizes and additional assay performance evaluations is needed before broader implementation.
Keywords: lymphatic filariasis, molecular sanoentoring real-time PCR Brugia meleyi Brupie timori Marsonia
INTERNATIONAL SCIENTIFIC CONFERENCE ON ENTOMOLOGY AND PEST OSCEP) 2005